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Autoimmunity pp 273-284 | Cite as

The Anti-DNA Knock-In Model of Systemic Autoimmunity Induced by the Chronic Graft-vs-Host Reaction

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Part of the Methods in Molecular Medicine™ book series (MIMM, volume 102)

Summary

The injection of spleen cells from bm12 mice into C57BL/6 recipients induces a chronic graft-vs-host reaction characterized by systemic autoimmunity, including anti-double-stranded DNA (anti-dsDNA) autoantibodies and immune complex-type proliferative glomerulonephritis. If the B6 recipient mice express an anti-DNA Vh site-directed transgene, the repertoire is skewed even more toward the anti-DNA response. Over a period of several weeks, high titers of serum anti-DNA antibodies appear and the mice develop renal damage. This permits the examination of the role of somatic immunoglobulin genetics and B-cell tolerance in a model of systemic lupus erythematosus.

Key Words

Anti-DNA autoimmunity B cells GVH SLE tolerance 
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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  1. 1.Division of Rheumatology, Department of MedicineUniversity of PennsylvaniaPhiladelphia

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