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Isolation of Photosystem I Reaction Center Subunit Polypeptides From Spinach

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Photosynthesis Research Protocols

Part of the book series: Methods In Molecular Biology™ ((MIMB,volume 274))

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Abstract

Methods to prepare photosystem (PS)I reaction center (RC) subunit polypeptides are described. For PsaC, PsaD, PsaE, and PsaY, the filtrate resulting from the ultrafiltration in Chapter 6 is used for preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). PsaA and PsaB, Type II PSI particles, prepared as described in the preceding chapter, are treated with sodium dodecyl sulfate, then applied on preparative SDS-PAGE. After electrophoresis, each band is cut out and electroeluted. A simple method to prepare PsaC is also presented.

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References

  1. Hiyama, T., Yumoto, K., Satoh, A., et al. (2000) Chromatographic separation of a small subunit (PsbW/PsaY) and its assignment to Photosystem I RC. Biochim. Biophys. Acta 1459, 117–124.

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  2. Anderson, J. M. and Boardman, N. K. (1966) Fractionation of the photochemical systems of photosynthesis I. Chlorophyll contents and photochemical activities of particles isolated from spinach chloroplasts. Biochim. Biophys. Acta 112, 403–421.

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© 2004 Humana Press Inc., Totowa, NJ

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Hiyama, T. (2004). Isolation of Photosystem I Reaction Center Subunit Polypeptides From Spinach. In: Carpentier, R. (eds) Photosynthesis Research Protocols. Methods In Molecular Biology™, vol 274. Humana Press. https://doi.org/10.1385/1-59259-799-8:063

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  • DOI: https://doi.org/10.1385/1-59259-799-8:063

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-232-2

  • Online ISBN: 978-1-59259-799-4

  • eBook Packages: Springer Protocols

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