Abstract
Primary murine splenic B cells can be cultured ex vivo and, following treatment with LPS, cytokines and/or CD40L proliferate, and undergo class switch recombination and terminal differentiation to become immunoglobulin-secreting plasmacytic cells. Methods are described here for introducing genes, encoding either normal or blocking forms of experimental proteins, into murine splenic B cells using retrovirus vectors. This makes it possible to study the effects of these proteins on late stages of B-cell development, including proliferation, class switch recombination, and plasmacytic differentiation.
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Lin, KI., Calame, K. (2004). Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors. In: Gu, H., Rajewsky, K. (eds) B Cell Protocols. Methods in Molecular Biology, vol 271. Humana Press. https://doi.org/10.1385/1-59259-796-3:139
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DOI: https://doi.org/10.1385/1-59259-796-3:139
Publisher Name: Humana Press
Print ISBN: 978-1-58829-347-3
Online ISBN: 978-1-59259-796-3
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