Abstract
Serine-threonine kinases play crucial roles in activating and inhibitory signaling pathways that control platelet activation. Diversity in isozyme composition, catalytic and regulatory subunits, and subcellular localization provide a multitude of signaling properties through which the platelet initiates its functional responses or prevents them. This chapter describes a number of techniques and assays that may be used to study various specific serine-threonine kinases in platelets and their regulation during the platelet-activation process.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Den Dekker, E., Gorter, G., Heemskerk, J. W. M., and Akkerman, J. W. N. (2002) Development of platelet inhibition by cAMP during megakaryocytopoiesis. J. Biol. Chem. 277, 29,321–29,329.
Kroner, C, Eybrechts, K., and Akkerman, J. W. N. (2000) Dual regulation of platelet protein kinase B. J. Biol. Chem. 275, 27,790–27,798.
Van Willigen, G. and Akkerman, J. W. N. (1992) Regulation of Glycoprotein IIB/IIIA exposure on platelets stimulated with α-thrombin. Blood 79, 82–90.
Hackeng, C. M., Relou, I., Pladet, M. W., van Rijn, H. J. M., and Akkerman, J. W. N. (1999) Early platelet activation by low density lipoprotein via p38 mapkinase. Thromb. Haemost. 82, 1749–1756.
Van Willigen, G., Gorter, G., and Akkerman, J. W. N. (2000) Thrombopoietin increases platelet sensitivity to α-thrombin via activation of the ERK2-cPLA2 pathway. Thromb. Haemost. 83, 610–616.
Den Dekker, E., Heemskerk, J. W. M., Gorter, G., van der Vuurst, H. de Jong-Donath, J., Kroner, C., et al. (2002) cAMP raises Ca2+ in human megakaryocytes independent of protein kinase A. Arterioscler. Thromb. Vasc. Biol. 22, 179–186.
Coffer, P. J., Jin, J., and Woodgett, J. R. (1998) Protein kinase B (c-Akt): a multifunctional mediator of phosphatidylinositol 3-kinase activation. Biochem. J. 335, 1–13.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Humana Press Inc.
About this protocol
Cite this protocol
Gorter, G., Akkerman, J.W. (2004). The Study of Serine-Threonine Kinases. In: Gibbins, J.M., Mahaut-Smith, M.P. (eds) Platelets and Megakaryocytes. Methods in Molecular Biology™, vol 273. Humana Press. https://doi.org/10.1385/1-59259-783-1:179
Download citation
DOI: https://doi.org/10.1385/1-59259-783-1:179
Publisher Name: Humana Press
Print ISBN: 978-1-58829-011-3
Online ISBN: 978-1-59259-783-3
eBook Packages: Springer Protocols