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In Vitro RNA Editing Systems From Higher Plant Chloroplasts

  • Tetsuro Hirose
  • Tetsuya Miyamoto
  • Junichi Obokata
  • Masahiro Sugiura
Part of the Methods in Molecular Biology book series (MIMB, volume 265)

Abstract

RNA editing in higher plant chloroplasts involves C-to-U conversion at ∼30 specific sites. In vitro systems supporting accurate editing have been developed from tobacco and pea chloroplasts. mRNA substrates labeled with 32P at C residues to be edited provide sensitive detection of editing activity in vitro. The present systems allow the rapid identification of cis-elements using mutated mRNA substrates and trans-acting factors by ultraviolet crosslinking.

Key Words

Chloroplast cis-acting element in vitro system pea RNA editing RNA ligation site-specific labeling tobacco trans-acting factor 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Tetsuro Hirose
    • 1
  • Tetsuya Miyamoto
    • 1
  • Junichi Obokata
    • 2
  • Masahiro Sugiura
    • 3
  1. 1.School of Biomedical ScienceTokyo Medical and Dental UniversityTokyoJapan
  2. 2.Center for Gene ResearchNagoya UniversityNagoyaJapan
  3. 3.Graduate School of Natural SciencesNagoya City UniversityNagoyaJapan

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