In Vitro RNA Editing Systems From Higher Plant Chloroplasts
RNA editing in higher plant chloroplasts involves C-to-U conversion at ∼30 specific sites. In vitro systems supporting accurate editing have been developed from tobacco and pea chloroplasts. mRNA substrates labeled with 32P at C residues to be edited provide sensitive detection of editing activity in vitro. The present systems allow the rapid identification of cis-elements using mutated mRNA substrates and trans-acting factors by ultraviolet crosslinking.
Key WordsChloroplast cis-acting element in vitro system pea RNA editing RNA ligation site-specific labeling tobacco trans-acting factor
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