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Posttranscriptional Gene Silencing in Plants

  • Susan Varsha Wesley
  • Chris Helliwell
  • Ming-Bo Wang
  • Peter Waterhouse
Part of the Methods in Molecular Biology book series (MIMB, volume 265)

Abstract

Double-stranded RNA when introduced into cells results in severe reduction of the target mRNA. This phenomenon is known as posttranscriptional gene silencing in plants and RNA interference in animals. Hairpin RNA-mediated gene silencing exploits this cellular mechanism. A convenient way of generating hairpin constructs is to use generic vectors such as pHANNIBAL and pHELLSGATE, vectors based on the Gateway® technology. These vectors are suitable for high-throughput gene silencing, and the silencing effect is stably inherited over many generations.

Key Words

Gene silencing RNA interference knockout hairpin RNA pHANNIBAL pHELLSGATE Gateway double-stranded RNA 

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Susan Varsha Wesley
    • 1
  • Chris Helliwell
    • 1
  • Ming-Bo Wang
    • 1
  • Peter Waterhouse
    • 1
  1. 1.CSIRO Plant IndustryCanberraAustralia

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