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Flow Cytometry Protocols pp 95–107Cite as

Cytokine Flow Cytometry

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 263))

Abstract

Cytokine flow cytometry (CFC) is a general term that applies to flow cytometric analysis of cells using anticytokine antibodies as markers of activation. The most common version of this technique is the intracellular staining of cytokines in cells that have been fixed and permeabilized after short-term in vitro activation. When used with specific antigens, this technique allows for the quantitation of rare populations of antigen-specific T cells. In this chapter, specific methodology for such intracellular staining is elaborated, with emphasis on the effects of variables such as sample type, antigens, activation conditions, sample processing, and data acquisition and analysis.

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Author information

Authors and Affiliations

  1. Immunocytometry Systems, BD Biosciences, San Jose, CA

    Holden T. Maecker

Authors
  1. Holden T. Maecker
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Editor information

Editors and Affiliations

  1. American Red Cross, Rockville, MD

    Teresa S. Hawley  & Robert G. Hawley  & 

  2. The George Washington University, Washington, DC

    Robert G. Hawley

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© 2004 Humana Press Inc.

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Cite this protocol

Maecker, H.T. (2004). Cytokine Flow Cytometry. In: Hawley, T.S., Hawley, R.G. (eds) Flow Cytometry Protocols. Methods in Molecular Biology™, vol 263. Humana Press. https://doi.org/10.1385/1-59259-773-4:095

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  • DOI: https://doi.org/10.1385/1-59259-773-4:095

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-234-6

  • Online ISBN: 978-1-59259-773-4

  • eBook Packages: Springer Protocols

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