Abstract
Generating gene-expression profiles from laser-captured cells requires the successful combination of laser-capture microdissection, RNA extraction, RNA amplification, and microarray analysis. To permit single-cell gene-expression profiling, the RNA amplification method has to be sufficiently powerful to bridge the gap between the amount of RNA available from a single cell to what is required by the microarray, a gap that spans 5 to 6 orders of magnitude. This chapter focuses on the amplification of RNA using a two-round T7 RNA amplification method. The protocols described are adapted for laser-captured material and have been used to generate gene expression profiles from single laser-captured cells.
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© 2004 Humana Press Inc.
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Kamme, F. et al. (2004). Single-Cell Laser-Capture Microdissection and RNA Amplification. In: Luo, Z.D. (eds) Pain Research. Methods in Molecular Medicine, vol 99. Humana Press. https://doi.org/10.1385/1-59259-770-X:099
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DOI: https://doi.org/10.1385/1-59259-770-X:099
Publisher Name: Humana Press
Print ISBN: 978-1-58829-103-5
Online ISBN: 978-1-59259-770-3
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