Abstract
Restoration of the balance of different ecological niches has been proposed as a way to control the income of pathogenic microorganisms (1). The genus Lactobacillus has been used in different human and animal tracts as probiotic microorganisms with this objective in mind (2). The characteristics of the strains proposed as probiotics have been published (3) or patented under the process of elaboration of different types of products (4–6). One of the mechanisms suggested to control the vaginal ecosystem is the production of antagonistic substances (lactic acid, bacteriocins, or H2O2) (7–9). The H2O2-producing microorganisms present in the vagina of healthy women have been suggested as some of the bacteria responsible for maintenance of ecological balance, mainly in pregnant women (10,11). The absence of these microorganisms is related to a higher risk of: bacterial vaginosis (12), recurrent urinary tract infections by Escherichia coli (13), and acquisition of human immunodeficiency virus type 1 (HIV-1) (14). Bauer (15) has proposed that H2O2-producing lactobacilli also might exert control over vaginal cancer through specific interactions of reactive oxygen species, such as superoxide anion, hydroxyl radicals, and hypochlorous acid. The conversion of H2O2 into more toxic compounds during the oxidative process is potentiated by peroxidase and halures. This enzyme and some halures, such as chloride and bromide, are present in vaginal washes in sufficient amounts (16) to allow an optimal environment for successful inhibition of pathogens.
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References
Reid, G. (1999) The scientific basis for probiotic strains of Lactobacillus. App. Env. Microbiol. 65, 3763–3766.
Redondo-López, V., Cook, R. L., and Sobel, J. D. (1990) Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora. Rev. Infect. Dis. 12, 856–872.
Reid, G. and Bruce, A. W. (2001) Selection of Lactobacillus strains for urogenital probiotic applications. J. Infect. Dis. 183(suppl 1), S77–S80.
Chrisope, G. L. (2000) Vaginal Lactobacillus medicant. US patent & trademark office, United States Patent, GyneLogix, Inc., Boulder, CO, No. 093,394-6-21.
Larsen, B. (1999) Vaginal pharmaceutical compositions. US patent & trademark office, United States Patent, Marshall University Research Corp. Huntington, WV, no. 5,958,461-7-34
Reid, G. and Bruce, A. W. (1997) Lactobacillus and skim milk compositions and methods for preventing microbial urogenital infections. US patent & trademark office, United States Patent, Research Corporation Technologies, Inc., Tucson, AZ, No. 5,643,830-1-1.
Eschenbach, D., Davick, P., Williams, S., Klebanoff, S., Young-Smith, C., and Holmes, K. K. (1989) Prevalence of hydrogen peroxide-producing Lactobacillus species in normal women with bacterial vaginosis. J. Clin. Microbiol. 27, 251–256.
Jack, R. W., Tagg, J. R., and Ray, B. (1995) Bacteriocins of Gram-positive bacteria. Microbiol. Rev. 59, 171–200.
Aroutcheva, A., Gariti, D., Simon, M., et al. (2001) Defense factors of vaginal lactobacilli. Am. J. Obstet. Gynecol. 185, 375–379.
McGroarty, J. (1993) Probiotic use of lactobacilli in the human female urogenital tract. FEMS Immunol. Med. Microbiol. 6, 251–264.
Goffeng, A. R., Holst, E., Milsom, I., Lindstedt, G., Lundberg, P. A., and Andersch, B. (1997) Fetal fibronectin and microorganisms in vaginal fluid of women with complicated pregnancies. Acta Obstet. Gynecol. Scand. 76, 521–527.
Hawes, S. E., Hillier, S. L., Benedetti, J., et al. (1996) Hydrogen peroxide-producing lactobacilli and acquisition of vaginal infections. J. Infec. Dis. 174, 1058–1063.
Gupta, K., Stapleton, A. E., Hooton, T. M., Roberts, P. L., Fennell, C. L., and Stamm, W. E. (1998) Inverse association of H2O2-producing lactobacilli and vaginal Escherichia coli colonization in women with recurrent urinary tract infections. J. Infect. Dis. 178, 446–450.
Martin, H. L. Jr., Richardson, B. A., Nyange, P. M., et al. (1999) Vaginal lactobacilli, microbial flora, and risk of human immunodeficiency virus type 1 and sexually transmitted disease acquisition. J. Infect. Dis. 180, 1863–1868.
Bauer, G. (2001) Lactobacilli-mediated control of vaginal cancer through specific reactive oxygen species interaction. Med. Hypotheses. 57, 252–257.
Klebanoff, S. J., Hillier, S. L., Eschenbach, D. A., Waltersdorph, A. M. (1991) Control of the microbial flora of the vagina by H2O2-generating lactobacilli. J. Infect. Dis. 164, 94–100.
Juven, B. J. and Pierson, M. D. (1996) Antibacterial effects of hydrogen peroxide and methods for its detection and quantification. J. Food Proteins 11, 1233–1241.
Berthier, F. (1993) On the screening of the hydrogen peroxide-generating lactic acid bacteria. Lett. Appl. Microbiol. 16, 150–153.
Nuñez de Kairuz, M. S., Olazábal, M. E., Oliver, G., de Ruiz Holgado, A. P., and Farías, R. N. (1988) Fatty acid-dependent hydrogen peroxide production in Lactobacillus. Biochem. Biophys. Res. Commun. 152, 113–121.
Ocaña, V. S., Bru, E., de Ruiz Holgado, A. P., and Nader-Macías, M. E. (1999) Surface characteristics of lactobacilli isolated from human vagina. J. Gen. Appl. Microbiol. 45, 203–212.
Otero, C., Silva de Ruiz, C., Ibáñez, R., Wilde, O., Ruiz Holgado, A. and Nader-Macías, M. E. (1999) Lactobacilli and enterococci isolated from the bovine vaginal during the estrous cycle. Anaerobe 5, 305–307.
Ocaña, V.S., P.de Ruiz Holgado, A., and Nader-Macías, M. E. (1999) Selection of vaginal H2O2-generating Lactobacillus for probiotic use. Curr. Microbiol. 38, 279–284.
Ocaña, V.S., P.de Ruiz Holgado, A., and Nader-Macías, M. E. (1999) Growth inhibition of Staphylococcus aureus by H2O2-producing Lactobacillus paracasei subsp. paracasei isolated from the human vagina. FEMS Immunol. Med. Microbiol. 23, 87–92.
Juárez Tomás, M. S., Bru, E., and Nader-Macías, M. E. (2002) Comparison of the growth and H2O2-production by vaginal probiotic lactobacilli under different culture conditions. Am. J. Obstet. Gynecol. 188, 35–44.
Marty-Teysset, C., De La Torre, F., Garel, J. R. (2000) Increased production of hydrogen peroxide by Lactobacillus delbrueckii subsp. bulgaricus upon aeration: Involvement of an NADH oxidase in oxidative stress. Appl. Env. Microbiol. 66, 262–267.
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© 2004 Humana Press Inc. Totowa, NJ
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Tomás, M.S.J., Otero, M.C., Ocaña, V., Nader-Macías, M.E. (2004). Production of Antimicrobial Substances by Lactic Acid Bacteria I. In: Spencer, J.F.T., Ragout de Spencer, A.L. (eds) Public Health Microbiology. Methods in Molecular Biology, vol 268. Humana Press. https://doi.org/10.1385/1-59259-766-1:337
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DOI: https://doi.org/10.1385/1-59259-766-1:337
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