Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy
Nuclear magnetic resonance (NMR) is a powerful technique to study protein-protein interactions in solution. Various methods have been developed and applied successfully for locating binding sites on proteins. The application of NMR chemical-shift perturbation to map the protein-protein interfaces is described in this chapter, providing a practical guideline that can be followed to carry out the experiments.
Key WordsNuclear magnetic resonance (NMR) protein-protein interaction chemical-shift perturbation heteronuclear single quantum correlation (HSQC) paramagnetic relaxation
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