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Protein-Protein Interaction Analysis by Nuclear Magnetic Resonance Spectroscopy

  • Guanghua Gao
  • Jason G. Williams
  • Sharon L. Campbell
Part of the Methods in Molecular Biology book series (MIMB, volume 261)

Abstract

Nuclear magnetic resonance (NMR) is a powerful technique to study protein-protein interactions in solution. Various methods have been developed and applied successfully for locating binding sites on proteins. The application of NMR chemical-shift perturbation to map the protein-protein interfaces is described in this chapter, providing a practical guideline that can be followed to carry out the experiments.

Key Words

Nuclear magnetic resonance (NMR) protein-protein interaction chemical-shift perturbation heteronuclear single quantum correlation (HSQC) paramagnetic relaxation 

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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Guanghua Gao
    • 1
  • Jason G. Williams
    • 2
  • Sharon L. Campbell
    • 1
  1. 1.Department of Biochemistry and BiophysicsUniversity of North Carolina-Chapel Hill
  2. 2.National Institute of Environmental Health SciencesResearch Triangle Park

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