Abstract
Protein microarrays permit the simultaneous measurement of many proteins in a small sample volume and therefore provide an attractive approach for the quantitative measurement of proteins in biological fluids, including serum. This chapter describes a microarray enzyme-linked immunosorbent assay (ELISA). Capture antibodies are immobilized onto a glass surface; the covalently attached antibodies bind a specific antigen from a sample overlaying the array. A second, biotinylated antibody that recognizes the same antigen as the first antibody, but at a different epitope, is then used for detection. Detection is based on an enzymatic signal-enhancement method known as tyramide signal amplification (TSA). By coupling a microarray-ELISA format with the signal amplification of tyramide deposition, the assay sensitivity is as low as sub-pg/mL.
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© 2004 Humana Press Inc., Totowa, NJ
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Varnum, S.M., Woodbury, R.L., Zangar, R.C. (2004). A Protein Microarray ELISA for Screening Biological Fluids. In: Fung, E.T. (eds) Protein Arrays. Methods in Molecular Biology, vol 264. Humana Press. https://doi.org/10.1385/1-59259-759-9:161
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DOI: https://doi.org/10.1385/1-59259-759-9:161
Publisher Name: Humana Press
Print ISBN: 978-1-58829-255-1
Online ISBN: 978-1-59259-759-8
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