Abstract
The authors describe a microarray system for disease diagnosis based on antibody-antigen interactions. Either biotinylated antibodies or antigens are coupled via streptavidin linkers onto a gold surface. This platform has been used to establish recombinant antibody-antigen interactions and to detect specific IgM antibodies in sera of patients suspected of Lyme borreliosis. Therefore, this microarray system can be adapted for further applications.
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© 2004 Humana Press Inc., Totowa, NJ
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Pavlickova, P., Hug, H. (2004). A Streptavidin-Biotin-Based Microarray Platform for Immunoassays. In: Fung, E.T. (eds) Protein Arrays. Methods in Molecular Biology, vol 264. Humana Press. https://doi.org/10.1385/1-59259-759-9:073
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DOI: https://doi.org/10.1385/1-59259-759-9:073
Publisher Name: Humana Press
Print ISBN: 978-1-58829-255-1
Online ISBN: 978-1-59259-759-8
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