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Receptor Signal Transduction Protocols pp 167–181Cite as

Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 259))

Abstract

Although methods for expressing foreign proteins in clonal cell lines are well established, mature neurons remain a difficult preparation for the introduction of foreign genes. Microinjection is a reliable method for producing robust targeted expression in neurons that has advantages over conventional transfection/infection methodologies. Here, I describe procedures for expressing signaling proteins in adult rat sympathetic neurons by direct microinjection of cRNA and cDNA into the cytoplasm and nucleus, respectively. The methods are applicable to a wide variety of peripheral and central neuron preparations, as well as clonal cell lines.

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Author information

Authors and Affiliations

  1. Laboratory of Molecular Physiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, MD

    Stephen R. Ikeda

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  1. Stephen R. Ikeda
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Editor information

Editors and Affiliations

  1. Department of Cell Physiology and Pharmacology, University of Leicester, Leicester, UK

    Gary B. Willars  & R. A. John Challiss  & 

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© 2004 Humana Press Inc.

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Ikeda, S.R. (2004). Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection. In: Willars, G.B., Challiss, R.A.J. (eds) Receptor Signal Transduction Protocols. Methods in Molecular Biology, vol 259. Humana Press. https://doi.org/10.1385/1-59259-754-8:167

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  • DOI: https://doi.org/10.1385/1-59259-754-8:167

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-329-9

  • Online ISBN: 978-1-59259-754-3

  • eBook Packages: Springer Protocols

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