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Determination of Kinetic Parameters for Hammerhead and Hairpin Ribozymes

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Part of the Methods in Molecular Biology™ book series (MIMB, volume 252)

Abstract

The application of conventional enzymological methods to the study of hairpin and hammerhead ribozymes has led to valuable insights into the mechanisms by which these small RNAs catalyze phosphodiester cleavage and ligation reactions. Here, protocols are presented for measuring rate constants for simple cleavage and ligation reactions mediated by minimal hammerhead and hairpin ribozymes under standard experimental conditions. Information is also provided to help researchers recognize and interpret more complex reaction kinetics that can be observed for ribozyme-sequence variants under a variety of reaction conditions.

Keywords

Cleavage Product Stop Solution Product Dissociation Hammerhead Ribozyme Hairpin Ribozyme 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  1. 1.Department of Molecular Biology and the Skaggs Institute for Chemical BiologyThe Scripps Research InstituteSan Diego

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