Abstract
The ability to regulate endogenous gene expression by cleaving mRNA is important in basic and applied biological research. This is now driven predominantly by small interfering RNAs (siRNAs) as they induce sequence-specific gene silencing. However, the major obstacle to the use of siRNAs as therapeutics is the difficulty involved in effective in vivo delivery. This chapter describes the liposomal delivery of siRNAs into adult mice.
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References
Sioud, M. (2001) Nucleic acid enzymes as a novel generation of anti-gene agents. Curr. Mol. Med. 1, 575–588.
Hannon, G. J. (2002) RNA interference. Nature 418, 244–251.
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., and Mello, C. C. (1998) Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391, 806–811.
Wianny, F. and Zernicka-Goetz, M. (2000) Specific interference with gene function by double-stranded RNA in early mouse development. Nature Cell Biol. 2, 70–75.
Elbashir, S. M., Harborth, J., Lendeckel, W., Yalcin, A., Weber, K., and Tuschl, T. (2001) Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411, 494–498.
Caplen, N. J., Parrish, S., Imani, F., Fire, A., and Morgen, R. A. (2001) Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems. Proc. Natl. Acad. Sci. USA 98, 9742–9747.
Sijen, T., Fleenor, J., Simmer, F., Thijssen, K. L., Parrish, S., Timmons, L., et al. (2001). On the role of RNA amplification in dsRNA-triggered gene silencing. Cell 107, 465–476.
Templeton, S. N. (2002) Liposomal delivery of nucleic acids in vivo. DNA Cell Biol. 21, 859–867.
Sørensen, D. R., Leirdal, M., and Sioud, M. (2003) Gene silencing by systemic delivery of synthetic siRNAs in adult mice. J. Mol. Biol. 327, 761–766
Kashani-Sabet, M., Liu, Y., Fong, S., Desprez, P.-Y., Liu, S., Tu, G., et al. (2002) Identification of gene function and functional pathways by systemic plasmid-based ribozyme targeting in adult mice. Proc. Natl. Acad. Sci. USA 99, 3878–3883.
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© 2004 Humana Press Inc., Totowa, NJ
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Sioud, M., Sørensen, D.R. (2004). Systemic Delivery of Synthetic siRNAs. In: Sioud, M. (eds) Ribozymes and siRNA Protocols. Methods in Molecular Biology™, vol 252. Humana Press. https://doi.org/10.1385/1-59259-746-7:515
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DOI: https://doi.org/10.1385/1-59259-746-7:515
Publisher Name: Humana Press
Print ISBN: 978-1-58829-226-1
Online ISBN: 978-1-59259-746-8
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