Abstract
Micron-sized fluid channels have been used for biology-related procedures, such as single-cell manipulation (1–3), cell deformation (4,5), cell assays (6), and subcellular molecule positioning (7). Owing to laminar fluid flow on this scale, small volumes of fluid can be precisely controlled (8,9). Microfluidic tools allow for the control and manipulation on the size scale of a single embryo (10). Precise microfluidic manipulation can be employed to perform multiple IVP-processing steps (11–13).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ayliffe, H. E., Rabbitt, R. D. Tresco, P. A., and Frazier, A. B. (1997) Micromachined cellular characterization system for studying the biomechanics of individual cells, in Transducers 97. International Conference on Solid-State Sensors and Actuators. Chicago, IL, IEEE, 1997.
Fuhr, G. and Shirley, S. G. (1995) Cell handling and characterization using micron and submicron electrode arrays: state of the art and perspectives of semiconductor microtools. J. Micromech. Microeng. 5, 77–85.
Li, P. C. H. and Harrison, D. J. (1997) Tranport, manipulation, and reaction of biological cells on-chip using electrokinetic effects. Anal. Chem. 69, 1564–1568.
Tracy, M. C., Greenway, R. S., Das, A.,. Kaye, P. H and Barnes, A. J. (1995) A silicon micromachined device for use in blood deformability studies. IEEE Transactions on Biomedical Engineering 42, 751–761.
Altendorf, E. (1997) Microfabrication-based ektacytometer for blood cell deformability measurements. SPIE 2978, 136–144.
Andersson, P. E., Li, P. C. H., Smith, R., Szarka, R. J., and Harrison, D. J. Biological cell assays on an electrokinetic microchip, in (1997 Int’l Conf. on Solid-State Sensors and Actuators. Chicago, IL, 1997.
Takayama, S., Ostuni, E., LeDuc, P., Naruse, K., Ingber, D., and G. M. (2001) Whitesides, Laminar flows: subcellular positioning of small molecules. Nature 411, 1016.
Brody, J. P., Yager, P., Goldstein, R. E., and Austin, R. (1996) Biotechnology at low reynolds numbers. Biophys. J. 71, 3430–3441.
Purcell, E. M. (1977) Life at low reynolds number. Am. J. Phys. 45, 3–11.
Glasgow, I., Zeringue, H. C., Beebe, D. J., Choi, S.-J., Lyman, J., Chan, N. G., and Wheeler, M. (2001) Handling individual mammalian embryos using microfluidics. IEEE Trans. Biomed. Eng. 48, 570–578.
Raty, S., Davis, J. A., Beebe, D. J., Rodriguez-Zas, S. L., and Wheeler, M. B. (2001) Culture in microchannels enhance in vitro embryonic development of pre-implantation mouse embryos. Theriogenology 55, 241.
Zeringue, H. C., Wheeler, M. R., and Beebe, D. J. (2001) Removal of cumulus from mammalian zygotes using micro fluidic techniques. Biomed. Microdevices 3, 219–224.
Zeringue, H. C., King, K. R., Glasgow, I. K., Raty, S., Wheeler, M. B. and Beebe, D. J. Zona pellucida removal of mammalian embryos in a microfluidic system, in Micro Total Analysis Systems. Enschede, the Netherlands, 2000.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Humana Press Inc.
About this protocol
Cite this protocol
Zeringue, H.C., Beebe, D.J. (2004). Microfluidic Removal of Cumulus Cells from Mammalian Zygotes. In: Schatten, H. (eds) Germ Cell Protocols. Methods in Molecular Biology™, vol 254. Humana Press. https://doi.org/10.1385/1-59259-741-6:365
Download citation
DOI: https://doi.org/10.1385/1-59259-741-6:365
Publisher Name: Humana Press
Print ISBN: 978-1-58829-257-5
Online ISBN: 978-1-59259-741-3
eBook Packages: Springer Protocols