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Live Imaging with Green Fluorescent Protein

  • Jim Haseloff
  • Emma-Louise Dormand
  • Andrea H. Brand
Part of the Methods in Molecular Biology™ book series (MIMB, volume 122)

Abstract

If developmental biologists were given the chance to design the perfect cell-or tissue-specific marker, they would ensure that it had several properties. First, it would function in living animals, eliminating the need for fixation and dehydration and their associated artefacts. Second, it would permit each stage of development to be studied in a single, intact embryo. Third, it would function in all cell types and would reveal their morphology, making it simple to identify different cells without compromising their viability.

Keywords

Green Fluorescent Protein Arabidopsis Seedling Green Fluorescent Protein Fluorescence Green Fluorescent Protein Fusion Vitelline Membrane 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Jim Haseloff
    • 1
  • Emma-Louise Dormand
    • 1
  • Andrea H. Brand
    • 1
  1. 1.Wellcome/CRC InstituteUniversity of CambridgeUK

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