Abstract
A prominent current paradigm in the discovery of potential drugs directed toward therapeutic targets relies on the combination of brute force and specificity. The increasing number of entities in pharmaceutical company compound libraries, frequently surpassing 106, dictates the use of a high-throughput screening (HTS) process. In practice, large numbers of individual compounds are quickly tested for their ability to interact with, and affect a change in, a previously identified and validated target protein. The heterologous expression of cloned receptor proteins in defined cell lines provides the specificity of the interaction.
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Cunningham, M.D., Cunningham, S.M. (1999). High-Throughput Functional Detection of NMDA Receptor Activity. In: Li, M. (eds) NMDA Receptor Protocols. Methods in Molecular Biology™, vol 128. Humana Press. https://doi.org/10.1385/1-59259-683-5:179
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DOI: https://doi.org/10.1385/1-59259-683-5:179
Publisher Name: Humana Press
Print ISBN: 978-0-89603-713-7
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