Abstract
Despite the availability of antibody libraries for the selection of receptor molecules, the large number of established and well-characterized hybridoma lines still represent a useful source for recombinant antibody genes. This protocol describes the PCR amplification, cloning, and a small-scale expression test for the generation of scFv fragments from hybridoma cell lines. Particular emphasis was placed on frequently observed problems and pitfalls of this method.
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Toleikis, L., Broders, O., Dübel, S. (2004). Cloning Single-Chain Antibody Fragments (scFv) from Hybridoma Cells. In: Decler, J., Reischl, U. (eds) Molecular Diagnosis of Infectious Diseases. Methods in Molecular Medicine™, vol 94. Humana Press. https://doi.org/10.1385/1-59259-679-7:447
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DOI: https://doi.org/10.1385/1-59259-679-7:447
Publisher Name: Humana Press
Print ISBN: 978-1-58829-221-6
Online ISBN: 978-1-59259-679-9
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