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Immunoprecipitation and Blotting

The Visualization of Small Amounts of Antigens Using Antibodies and Lectins
  • Stephen Thompson
Part of the Methods in Molecular Medicine™ book series (MIMM, volume 94)

Abstract

The practical problems encountered when purifying and visualizing small amounts of antigens from complex cellular and protein mixtures are explored. Practical aspects and the relative advantages and disadvantages of immunoprecipitation and blotting, the two most commonly used antibody techniques, are discussed. As glycosylation of antigens is becoming recognized as an important factor in the progress of many diseases, a short section on the use of lectins in precipitation and blotting techniques is also included. It is highly likely that a combination of precipitation followed by blotting, using either lectin followed by antibodies or antibody followed by lectins, will become a valuable tool in characterizing cellular antigens and the progression of disease.

Keywords

Sodium Dodecyl Sulfate Wheat Germ Agglutinin Protein Mixture Sepharose Bead Metabolic Label 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Stephen Thompson
    • 1
  1. 1.Department of Clinical BiochemistryUniversity of Newcastle upon Tyne, The Medical SchoolNewcastle upon TyneUK

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