Molecular Diagnosis of Infectious Diseases pp 159-175

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 94) | Cite as

Application of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial Components

  • Ludwig Deml
  • Michael Aigner
  • Alexander Eckhardt
  • Jochen Decker
  • Norbert Lehn
  • Wulf Schneider-Brachert

Abstract

Recently, various bacterial components have been suggested as initiating and modulating immune activation, thereby substantially affecting the complex and dynamic host/pathogen interactions. Herein, we present a valuable and simple methodology for determining the capacity of bacteria as well as defined bacterial structures to stimulate cellular effectors of the innate and cognate immune system. This assay format is based on the exposure of freshly prepared single-cell cultures of splenic cells derived from naive mice with the immunogen of interest. Herein, the determination of exclusive panels of cytokines by the ELISA, ELISpot, and FACS technology will serve as an indicator for the activation of defined arms of the immune system. An increased knowledge about microbial components with immunomodulatory properties will substantially contribute to a more detailed understanding of the dynamic interplay between the host and potential pathogens and, based on this knowledge, to the development of novel substances for the prevention and therapy of microbial infections.

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Copyright information

© Humana Press Inc. 2004

Authors and Affiliations

  • Ludwig Deml
    • 1
  • Michael Aigner
    • 1
  • Alexander Eckhardt
    • 1
  • Jochen Decker
    • 2
  • Norbert Lehn
    • 1
  • Wulf Schneider-Brachert
    • 1
  1. 1.Institute for Medical Microbiology and HygieneUniversity of RegensburgRegensburgGermany
  2. 2.Accenture GmbHMunichGermany

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