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In Situ Hybridization Protocols pp 83–102Cite as

In Situ Hybridization to Polytene Chromosomes of Drosophila melanogasterand Other Dipteran Species

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 123))

Abstract

In situ hybridization of nucleotide sequence to Drosophila melanogaster interphase polytene chromosomes was initially developed by Pardue, who has published an extensive account of Drosophila polytene chromosomes and hybridization to these chromosomes (1). The procedure, which used radioactively labeled probes, was later adapted to allow the use of more sensitive and safer nonradioactive labeling methods (2). The method has been used extensively for gene mapping and identification of transposable element insertion sites. In situ hybridization to chromosomes has also been performed in cross-species studies within Diptera to give valuable evolutionary information, including conservation of linkage groups, and to identify homologous genes in related species (3-5). In addition, there have been recent advances that extend this technique to salivary gland preparations from stored fixed specimens (6).

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Author information

Authors and Affiliations

  1. Department of Genetics, The University of Melbourne, Parkville, Victoria, Australia

    A. Marie Phillips & Jon Martin

  2. Rural Division, Department of Primary Industries and Energy, Canberra, ACT, Australia

    Daniel G. Bedo

Authors
  1. A. Marie Phillips
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  2. Jon Martin
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  3. Daniel G. Bedo
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Editor information

Editors and Affiliations

  1. RMIT University Bundoora, Victoria, Australia

    Ian A. Darby

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© 2000 Humana Press Inc.

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Phillips, A.M., Martin, J., Bedo, D.G. (2000). In Situ Hybridization to Polytene Chromosomes of Drosophila melanogasterand Other Dipteran Species. In: Darby, I.A. (eds) In Situ Hybridization Protocols. Methods in Molecular Biology™, vol 123. Humana Press. https://doi.org/10.1385/1-59259-677-0:83

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  • DOI: https://doi.org/10.1385/1-59259-677-0:83

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-686-4

  • Online ISBN: 978-1-59259-677-5

  • eBook Packages: Springer Protocols

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