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Studying DHBV Polymerase by In Vitro Transcription and Translation

  • Protocol
Hepatitis B and D Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 95))

Abstract

Duck hepatitis B virus (DHBV), like all other hepadnaviruses, is a retroid virus that bears a small DNA genome and replicates this DNA genome via reverse transcription through an RNA intermediate called pregenomic RNA (pgRNA; 1). All hepadnaviruses encode a multifunctional polymerase (pol), which is a specialized reverse transcriptase (RT) and plays key roles in several different aspects of the viral life cycle (2–5). Pol has an RNA- and DNA-directed DNA polymerase (i.e., RT) activity that is responsible for DNA genome replication, which also entails its intrinsic RNase H activity responsible for degrading the pgRNA as the pgRNA is being reverse transcribed into the first (minus) strand of the DNA genome. In addition to the RT and RNase H activities, which are shared with retroviral RTs, the hepadnavirus polymerase displays the unique ability to initiate DNA synthesis de novo using pol itself as a protein primer (protein priming) (6–9).

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Authors and Affiliations

  1. Department of Microbiology, Boston University School of Medicine, Boston, MA

    Jianming Hu

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  1. Jianming Hu
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Editors and Affiliations

  1. Ribapharm Inc., Costa Mesa, CA

    Robert K. Hamatake PhD  & Johnson Y. N. Lau MD  & 

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© 2004 Humana Press Inc.

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Hu, J. (2004). Studying DHBV Polymerase by In Vitro Transcription and Translation. In: Hamatake, R.K., Lau, J.Y.N. (eds) Hepatitis B and D Protocols. Methods in Molecular Medicine, vol 95. Humana Press. https://doi.org/10.1385/1-59259-669-X:259

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  • DOI: https://doi.org/10.1385/1-59259-669-X:259

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-105-9

  • Online ISBN: 978-1-59259-669-0

  • eBook Packages: Springer Protocols

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