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A One-Filter–Three-Probe Assay for Defective Interference (DI) Effects of Naturally Occurring Core Internal Deletion (CID) Variants of Human Hepatitis B Virus

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Book cover Hepatitis B and D Protocols

Part of the book series: Methods in Molecular Medicine ((MIMM,volume 95))

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Abstract

“Incomplete particles” were discovered during successive undiluted passages of the influenza viruses (1). In general, these incomplete particles contain a less than full-length genome and are replication-defective. They can be rescued by, and interfere with, the replication of homologous helper viruses. Another important characteristic of incomplete particles is their ability to enrich their proportion in the total viral yield in cells infected with wild-type and incomplete viruses (2–4). Based on these properties, Huang and Baltimore defined these biologically active incomplete particles as defective interfering (DI) particles and the replication-competent homologous helper viruses as standard viruses (5).

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Author information

Authors and Affiliations

  1. Departments of Pathology, and Microbiology & Immunology, WHO Collaborating Center for Tropical Diseases and Sealy Center for Vaccine Developments, University of Texas Medical Branch, Galveston, TX

    Chiaho Shih & Ta-Tung Thomas Yuan

Authors
  1. Chiaho Shih
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  2. Ta-Tung Thomas Yuan
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Editors and Affiliations

  1. Ribapharm Inc., Costa Mesa, CA

    Robert K. Hamatake PhD  & Johnson Y. N. Lau MD  & 

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© 2004 Humana Press Inc.

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Shih, C., Yuan, TT.T. (2004). A One-Filter–Three-Probe Assay for Defective Interference (DI) Effects of Naturally Occurring Core Internal Deletion (CID) Variants of Human Hepatitis B Virus. In: Hamatake, R.K., Lau, J.Y.N. (eds) Hepatitis B and D Protocols. Methods in Molecular Medicine, vol 95. Humana Press. https://doi.org/10.1385/1-59259-669-X:151

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  • DOI: https://doi.org/10.1385/1-59259-669-X:151

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-105-9

  • Online ISBN: 978-1-59259-669-0

  • eBook Packages: Springer Protocols

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