Abstract
Detection of minute amounts of hepatitis B virus (HBV) DNA in the serum using polymerase chain reaction (PCR)-based assay involves extracting the viral DNA from the viral particle in the serum, removing inhibitors of PCR, performing the PCR, and detecting the PCR product. PCR is an extremely sensitive assay, and preventing cross contamination is an important part of the assay.
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© 2004 Humana Press Inc.
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Chung, H.T. (2004). Detection of HBV DNA in Serum Using a PCR-Based Assay. In: Hamatake, R.K., Lau, J.Y.N. (eds) Hepatitis B and D Protocols. Methods in Molecular Medicine, vol 95. Humana Press. https://doi.org/10.1385/1-59259-669-X:15
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DOI: https://doi.org/10.1385/1-59259-669-X:15
Publisher Name: Humana Press
Print ISBN: 978-1-58829-105-9
Online ISBN: 978-1-59259-669-0
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