Abstract
Leukocytes play an important role in inflammation and cancer. They are attracted to the place of injury by chemotactic factors, e.g., plasma complement factors C5a and C3a, and chemotactic cytokines or chemokines, which are produced locally at the site of infection. Chemokines are produced by a variety of cell types including tumor cells. Leukocytes, selectively attracted by chemokines, will modulate the inflammatory response (phagocytosis) and affect tissue remodeling and tumorigenesis by the chemokine-induced release of proteases and other metabolites (1).
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References
Opdenakker, G. and Van Damme, J. (1992) Cytokines and proteases in invasive processes: molecular similarities between inflammation and cancer. Cytokine 4, 251–258.
Wang, J. M., Chertov, O., Proost, P., et al. (1998) Purification and identification of chemokines potentially involved in kidney-specific metastasis by a murine lymphoma variant: induction of migration and NFkB activation. Int. J. Cancer 75, 900–907.
Boyden, S. (1962) The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J. Exp. Med. 115, 453–466.
Falk, W., Goodwin, R. H., Jr., and Leonard, E. J. (1980) A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration. J. Immunol. Methods 33, 239–247.
Nelson, R. D., Quie, P. G., and Simmons, R. L. (1975) Chemotaxis under agarose: a new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes. J. Immunol. 115, 1650–1656.
Van Osselaer, N., Van Damme, J., Rampart, M., and Herman, A. G. (1991) Increased microvascular permeability in vivo in response to intradermal injection of neutrophil-activating protein (NAP-2) in rabbit skin. Am. J. Pathol. 138, 23–27.
Wuyts, A., Proost, P., and Van Damme, J. (1998) Interleukin-8 and other CXC chemokines, in The Cytokine Handbook, 3rd ed. (Thomson, A. W., ed.), Academic, London, pp. 271–311.
Proost, P., Wuyts, A., and Van Damme, J. (1996) Human monocyte chemotactic proteins-2 and-3: structural and functional comparison with MCP-1. J. Leukoc. Biol. 59, 67–74.
Alam, R., Forsythe, P., Stafford, S., Heinrich, J., Bravo, R., Proost, P., and Van Damme, J. (1994) Monocyte chemotactic protein-2, monocyte chemotactic protein-3, and fibroblast-induced cytokine. Three new chemokines induce chemotaxis and activation of basophils. J. Immunol. 153, 3155–3159.
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© 2004 Humana Press Inc., Totowa, NJ
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Wuyts, A., Menten, P., Van Osselaer, N., Van Damme, J. (2004). Assays for Chemotaxis. In: De Ley, M. (eds) Cytokine Protocols. Methods in Molecular Biology, vol 249. Humana Press. https://doi.org/10.1385/1-59259-667-3:153
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DOI: https://doi.org/10.1385/1-59259-667-3:153
Publisher Name: Humana Press
Print ISBN: 978-0-89603-948-3
Online ISBN: 978-1-59259-667-6
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