Making and Changing Buffers

  • Shawn Doonan
Part of the Methods in Molecular Biology book series (MIMB, volume 244)


Control of pH is a central consideration in handling proteins, and this requires the use of buffer solutions. Furthermore, a typical protein purification schedule will contain several steps generally needed to be carried out in buffers of different pH values and ionic strengths (see Note 1). Hence, it is necessary to have available methods for changing buffers. The present chapter reviews the properties of buffers and preparation of buffer solutions and then deals with ways in which a protein solution can be changed from one buffer to another.


Protein Solution Column Volume Protein Mixture Neutral Salt External Buffer 
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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Shawn Doonan
    • 1
  1. 1.School of BiosciencesUniversity of East LondonUK

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