Making and Changing Buffers
Control of pH is a central consideration in handling proteins, and this requires the use of buffer solutions. Furthermore, a typical protein purification schedule will contain several steps generally needed to be carried out in buffers of different pH values and ionic strengths (see Note 1). Hence, it is necessary to have available methods for changing buffers. The present chapter reviews the properties of buffers and preparation of buffer solutions and then deals with ways in which a protein solution can be changed from one buffer to another.
KeywordsProtein Solution Column Volume Protein Mixture Neutral Salt External Buffer
- 1.Dawson, R. M. C., Elliot, D. C., Elliot, W. H., and Jones, K. M. (1986) Data for Biochemical Research, 3rd ed., Oxford University Press, Oxford, pp. 417–448.Google Scholar