Abstract
Plant tissues contain a wide range of proteins, which vary greatly in their properties, and require specific conditions for their extraction and purification. It is therefore not possible to recommend a single protocol for extraction of all plant proteins.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Beccari, J. B. (1745) De Frumento, in De Bononiensi Scientiarum et Artium atque Academia Commentarii, Tomi Secundi. Bononia, Bologna.
Matschke, O. (1859) Ueber den Bau und die Bestandtheile der Kleberbläschen in Bertholletia, deren Entwickelung in Ricinus, nebst einigen Bemerkunger über Amylonbläschen. Botan. Z. 17, 409–447.
Danielsson, C. E. (1949) Seed globulins of the Gramineae and Leguminosae. Biochem. J. 44, 387–400.
Osborne, T. B. (1924) The Vegetable Proteins, Longmans Green, London.
Wray, J. L. and Fido, R. J. (1990) Nitrate and nitrite reductase, in Methods in Plant Biochemistry (Lea, P. J., ed.), Academic, New York, Vol. 3, pp. 241–256.
Keys, A. J. and Parry, M. A. J. (1990) Ribulose bisphosphate carboxylase/oxygenase and carbonic anhydrase, in Methods in Plant Biochemistry (Lea, P. J., ed.), Academic, New York, Vol. 3, pp. 1–14.
Somers, D. A., Kuo, T.-M., Kleinhofs, A., Warner, R. L., and Oaks, A. (1983) Synthesis and degradation of barley nitrate reductase. Plant Physiol. 72, 949–952.
Shewry, P. R., Franklin, J., Parmar, S., Smith, S. J., and Miflin, B. J. (1983) The effects of sulphur starvation on the amino acid and protein compositions of barley grain. J. Cereal Sci. 1, 21–31.
Nelson, T., Harpster, M. H., Mayfield, S. P., and Taylor, W. C. (1984) Light regulated gene-expression during maize leaf development. J. Cell. Biol. 98, 558–564.
Bjorksten, F, Halmepuro, L., Hannuksela, M., and Lahti, A. (1980) Extraction and properties of apple allergens. Allergy 35, 671–677.
Servaites, J. C, Parry, M. A. J, Gutteridge, S., and Keys, A. J. (1986) Species variation in the predawn inhibition of ribulose-1,5-bisphosphate carboxylase/oxygenase. Plant Physiol. 82, 1161–1163.
Maxwell, K, Borland, A. M., Haslam, R. P., Helliker, B. R., Roberts, A. and Griffiths, H. (1999) Modulation of Rubisco activity during the diurnal phases of the Crassulacean acid metabolism plant Kalanchoe daigremontiana. Plant Physiol. 121, 849–856.
Nason, A. (1955) Extraction of soluble enzymes from higher plants. Methods Enzymol. 1, 62–63.
Green, A. A. and Hughes, W. L. (1955) Protein fractionation on the basis of solubility in aqueous solutions of salts and organic solvents. Methods Enzymol. 1, 67–90.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2004 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Fido, R.J., Mills, E.N.C., Rigby, N.M., Shewry, P.R. (2004). Protein Extraction From Plant Tissues. In: Cutler, P. (eds) Protein Purification Protocols. Methods in Molecular Biology, vol 244. Humana Press. https://doi.org/10.1385/1-59259-655-X:21
Download citation
DOI: https://doi.org/10.1385/1-59259-655-X:21
Publisher Name: Humana Press
Print ISBN: 978-1-58829-067-0
Online ISBN: 978-1-59259-655-3
eBook Packages: Springer Protocols