Bulk Purification by Fractional precipitation

  • Shawn Doonan
Part of the Methods in Molecular Biology book series (MIMB, volume 244)

Abstract

The solubility of a particular protein in aqueous solution depends on the solvent composition and on the pH; hence, variation in these parameters provides a way of purifying proteins by fractional precipitation. The factors that dictate solubility are complex, because the surface of a protein is itself complex, containing ionized residues, polar regions, and hydrophobic patches, all of which will interact with the solvent in ways that are not completely understood. Hence, it is not possible to elaborate a theoretical approach to fractional precipitation (1); rather, the methods are used in an essentially empirical fashion. The most widely used procedures are precipitation by the addition of salt (salting out) and by the addition of organic solvents; these are the focus of this chapter (see Note 1).

Keywords

Protein Solution Ammonium Sulfate Precipitate Protein Dialysis Tubing Fractional Precipitation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Shawn Doonan
    • 1
  1. 1.School of BiosciencesUniversity of East LondonUK

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