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Separation of Enantiomers by Thin-Layer Chromatography

An Overview

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Chiral Separations

Part of the book series: Methods in Molecular Biology ((MIMB,volume 243))

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Abstract

The present review discusses the versatile applicability and separation mechanisms of thin-layer chromatographic enantiomeric separations. More detailed descriptions will be given for practical applications—separations of underivatized samples—on commercially available, ready-to-use plates, focusing on the thin-layer chromatographic racemate separation based on ligand exchange, which was introduced in 1983 by Günther et al. (1) and on use of a densitometer for determination of antipode distributions at trace level. This chapter will not discuss the numerous and interesting diastereomeric separations by paper and thin-layer chromatography (TLC). We refer to the literature on amino acids (2–8), peptides, diketopiperazines (9–18), and other classes of compounds (19–31).

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Günther, K., Richter, P., Möller, K. (2004). Separation of Enantiomers by Thin-Layer Chromatography. In: Gübitz, G., Schmid, M.G. (eds) Chiral Separations. Methods in Molecular Biology, vol 243. Humana Press. https://doi.org/10.1385/1-59259-648-7:029

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  • DOI: https://doi.org/10.1385/1-59259-648-7:029

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-150-9

  • Online ISBN: 978-1-59259-648-5

  • eBook Packages: Springer Protocols

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