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Growth Cones of Living Neurons Probed by Atomic Force Microscopy

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Part of the Methods in Molecular Biology™ book series (MIMB,volume 242)

Abstract

A large body of recent literature describes the use of atomic force microscopy (AFM; ref. 1) for the study of living cells. These experimental findings clearly indicate that AFM is a very valuable tool for the 3D imaging of flat biological samples strongly adhering to a substrate, with a lateral resolution in between the resolutions of optical and electron microscopy. Moreover, a very relevant feature of AFM is its capability of analyzing local mechanical properties of living cells.

Keywords

  • Silicon Nitride
  • Growth Cone
  • Spinal Cord Neuron
  • Neuron Cell Culture
  • Indentation Curve

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Binning, G., Quate, C. F., and Gerber, C. (1986) Atomic force microscope. Phys. Rev. Lett. 56, 930–933.

    CrossRef  Google Scholar 

  2. Schoenenberger C.-A. and Hoh, J. H. (1994) Slow cellular dynamics in MDCK and R5 cells monitored by time-lapse atomic force microscopy. Biophys. J. 67, 929–936.

    PubMed  CrossRef  CAS  Google Scholar 

  3. Hoh, J. H. and Schoenenberger, C.-A. (1994) Surface morphology and mechanical properties of MDCK monolayers by atomic force microscopy. J. Cell Sci. 107, 1105–1114.

    PubMed  Google Scholar 

  4. Ricci, D., Tedesco, M., and Grattarola, M. (1997) Mechanical and morphological properties of living 3t6 cells probed via scanning force microscopy. Microsc. Res. Tech. 36, 165–171.

    PubMed  CrossRef  CAS  Google Scholar 

  5. Henderson, E., Haydon, P. G., and Sakaguchi, D. S. (1992) Actin filaments dynamics in living glial cells imaged by atomic force microscopy. Science 257, 1944–1946.

    PubMed  CrossRef  CAS  Google Scholar 

  6. Parpura, V., Haydon, P., and Henderson, E. (1993) Three-dimensional imaging of living neurons and glia with the atomic force microscope. J. Cell Sci. 104, 427–432.

    PubMed  Google Scholar 

  7. Ricci, D. and Grattarola M. (1994) Scanning force microscopy on live cultured cells: Imaging and force-versus-distance investigations. J. Microsc. 176, 254–261.

    PubMed  CAS  Google Scholar 

  8. Butt, H.-J., Siedle P., Seifert K., Fendler K., Seeger T., Bamberg E., et al. (1993) Scan speed limit in atomic force microscopy. J. Microsc. 169, 75–84.

    Google Scholar 

  9. Putman, C. A. J., van der Werf. K.O., de Grooth, B. G., van Hulst, N. F., Greve, J., and Hansma, P. K. (1992) A new imaging mode in atomic force microscopy based on the error signal. Proc. SPIE. 1639, 198–204.

    CrossRef  Google Scholar 

  10. Sneddon, J. N. (1965) The relation between load and penetration in the axisymmetric Boussinesq problem for a punch of arbitrary profile. Int. J. Eng. Sci. 3, 47–57.

    CrossRef  Google Scholar 

  11. Lewis, A. K. and Bridgam P. C. (1992) Nerve growth cone lamellipodia contain two populations of actin filaments that differ in organization and polarity. J. Cell. Biol. 119, 1219–1243.

    PubMed  CrossRef  CAS  Google Scholar 

  12. Da-Yu, W. and Golberg, D. J. (1993) Regulated tyrosine phosphorylation at the tips of growth cone filopodia. J. Cell Biol. 123, 653–664.

    CrossRef  Google Scholar 

  13. Hoh, J. H., Sosinsky, G. E., Revel, J.-P., and Hansma, P. K. (1993) Structure of the extracellular surface of the gap junction by atomic force microscopy. Biophys. J. 65, 149–163.

    PubMed  CrossRef  CAS  Google Scholar 

  14. Bridgman, P. C. and Dailey M. E. (1989) The organization of myosin and actin in rapid frozen nerve growth cones. J. Cell Biol. 108, 95–109.

    PubMed  CrossRef  CAS  Google Scholar 

  15. Bridgman, P. C. (1991) Functional anatomy of the growth cone in relation to its role in locomotion and neurite assembly, in The Nerve Growth Cone (Letourneau, P. C., Kater, S. B., and Macagno E. R., eds.), Raven Press, New York, pp. 39–53.

    Google Scholar 

  16. Gordon-Weeks, P. R. and Mansfield G. S. (1991) Assembly of microtubules in growth cones: the role of microtubule-associated proteins, in The Nerve Growth Cone (Letourneau, P. C., Kater, S. B., and Macagno E. R., eds.), Raven Press, Ne York, pp. 55–64.

    Google Scholar 

  17. Goldberg, D. J., Burmeister, D. W., and Rivas, R. J. (1991) Video microscopic analysis of events in the growth cone underlying axon growth and the regulation of these events by substrate-bound proteins, in The Nerve Growth Cone (Letourneau, P. C., Kater, S. B., and Macagno E. R., eds.), Raven Press, New York, pp. 79–95.

    Google Scholar 

  18. Dai, J. and Sheetz, M. P. (1995) Mechanical properties of neuronal growth cone membranes studied by tether formation with laser optical tweezers. Biophys. J. 68, 988–996.

    PubMed  CrossRef  CAS  Google Scholar 

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© 2004 Humana Press Inc., Totowa, NJ

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Ricci, D., Grattarola, M., Tedesco, M. (2004). Growth Cones of Living Neurons Probed by Atomic Force Microscopy. In: Braga, P.C., Ricci, D. (eds) Atomic Force Microscopy. Methods in Molecular Biology™, vol 242. Humana Press. https://doi.org/10.1385/1-59259-647-9:125

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  • DOI: https://doi.org/10.1385/1-59259-647-9:125

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-094-6

  • Online ISBN: 978-1-59259-647-8

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