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Protocols for Anther and Microspore Culture of Barley

Protocol
Part of the Methods In Molecular Biology™ book series (MIMB, volume 111)

Abstract

The establishment of true breeding lines is a critical step for variety development in most crop plants. Traditionally, plant breeders have achieved homozygosity for all genes by using the time- and labor-intensive methods of self-fertilization or backcrossing. The phenomenon of parthenogenesis allows the use of androgenetic in vitro methods in order to shorten the time needed for the production of homozygous plants. It is possible to switch microspore development from the normal gametophytic pathway into the sporophytic pathway. The process of microspore embryogenesis makes it possible to regenerate homozygous plants originating from single cells. In barley, there is a high percentage—up to 90% dependent on the genotype—of dihaploid regenerants owing to a single autoendoreduplication of the genome during the first division of the microspore. Accordingly, there is no need for an application of agents, such as, e.g., colchicine, to induce chromosome doubling.

Keywords

Anther Culture Flag Leaf Donor Plant Microspore Culture Microspore Embryogenesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  1. 1.Angewandte Molekularbiologie der PflanzenInstitut fuer Allgemeine BotanikHamburgGermany

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