Advertisement

Protoplast Isolation, Culture, and Plant Regeneration from Passiflora

Protocol
Part of the Methods In Molecular Biology™ book series (MIMB, volume 111)

Abstract

The family Passifloraceae contains over 580 woody or herbaceous species (1), the majority of species within the genus Passiflora being found in tropical South America. Passiflora eduhs fv. flavicarpa is considered to be the most important species (2) because of its value in the fruit juice industry. Additionally, this species is resistant to the soil-borne pathogen Fusarium oxysporum and, consequently, is frequently used as a rootstock onto which is grafted P edulis Sims. Interspecific sexual hybridization has been attempted in Passiflora breeding programs, using wild-type germplasms to transfer disease resistance and other potentially desirable traits into cultivated species. However, fertile hybrids have been difficult to obtain (3). Somatic hybridization provides a means of circumventing such sexual incompatibilities. Indeed, novel fertile somatic hybrids have been produced between P. eduhs fv. flavicarpa and Passiflora incarnata (4). Since the latter species can survive winter temperatures of −16°C, such transfer of cold tolerance to the commercial crop would permit cultivation in more temperate climates (5). Somatic hybrid plants have also been produced between P edulis fv. flavicarpa and Passiflora amethystina, Passiflora cincinnata, Passiflora giberti, and Passiflora alata, respectively (6).

Keywords

Somatic Hybrid Nylon Sieve Somatic Hybrid Plant Daylight Fluorescent Tube Seaplaque Agarose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in to check access.

References

  1. 1.
    Oliviera, J. C (1987) Melhoramento genetico in Cultura do maracujazeiro, vol 1 (Ruggiero, C, ed.), Editora Legis Summa, Ribeirão Petro, pp 218–246.Google Scholar
  2. 2.
    Vanderplank, J. (1991) Passion Flowers, Cassel Publishers, London.Google Scholar
  3. 3.
    Payan, F. R, and Martin, F. W. (1975) Barriers to the hybridization of Passiflora species Euphytica 24, 709–716CrossRefGoogle Scholar
  4. 4.
    Otoni, W C, Blackhall, N W, ďUtra Vaz, F. B., Casah, V. W., Power, J. B., and Davey, M. R. (1995) Somatic hybridization of the Passiflora species, Passiflora edulis fv flavicarpa Degener and P incarnata L J Exp Bot 46, 777–785Google Scholar
  5. 5.
    Dozier, W. A, Jr., Rodriguez-Kabana, R., Caylor, A W, Himelnck, D. G, McDaniel, N R, and McGuire, J. A. (1991) Ethephon hastens maturity of passionfruit grown as an annual in a temperate zone Hort Sci 26, 146–147.Google Scholar
  6. 6.
    Dornelas, M C, Tavares, F. C A., Oliviera, J C., and Vieira, M L C (1995) Plant regeneration from protoplast fusion in Passiflora spp. Plant Cell Rep 15, 106–110CrossRefGoogle Scholar
  7. 7.
    ďUtra Vaz, F B, dos Santos, A V P, Manders, G, Cocking, E. C, Davey, M R., and Power, J. B (1993) Plant regeneration from leaf mesophyll protoplasts of the tropical woody plant, passionfruit (Passiflora edulis fv flavicarpa Degener) the importance of the antibiotic cefotaxime in the culture medium. Plant Cell Rep. 12, 220–225Google Scholar
  8. 8.
    Dornelas, M. C. and Vieira, M. L. C. (1993) Plant regeneration from protoplast cultures of Passiflora edulis var flavicarpa Deg., P amethystina Mikan. and P cincinnata Mast Plant Cell Rep 13, 103–106CrossRefGoogle Scholar
  9. 9.
    Frearson, E. M., Power, J B., and Cocking, E C (1973) The isolation, culture and regeneration of Petunia protoplasts Dev. Biol. 33, 130–137.PubMedCrossRefGoogle Scholar
  10. 10.
    Kao, K. N. and Michayluk, M. R. (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media Planta 126, 105–110.CrossRefGoogle Scholar
  11. 11.
    Gilmour, D M, Golds, T. J., and Davey, M. R. (1989) Medwago protoplasts. fusion, culture and plant regeneration, in Biotechnology in Forestry and Agriculture, vol 8, Plant Protoplasts and Genetic Engineering I (Bajaj, Y. P. S, ed), Springer-Verlag, Heidelberg, pp. 370–388.Google Scholar
  12. 12.
    Widholm, J. (1972) The use of FDA and phenosafranine for determining viability of cultured plant cells. Stain Technol 47, 186–194Google Scholar
  13. 13.
    Murashige, T. and Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 56, 473–497CrossRefGoogle Scholar
  14. 14.
    Muller, A. J. and Grafe, R. (1978) Isolation and characterisation of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol Gen Genet 161, 67–76.CrossRefGoogle Scholar
  15. 15.
    Jain, R. K., Khehra, G S, Lee, S-H., Blackhall, N. W., Marchant, R, Davey, M R, et al. (1995) An improved procedure for plant regeneration from indica and japonica rice protoplasts. Plant Cell Rep. 14, 515–519.CrossRefGoogle Scholar
  16. 16.
    Thompson, J. A., Abdullah, R., and Cocking, E C. (1986) Protoplast culture of rice (Oryza sativa L.) using media solidified with agarose Plant Sci 47, 179–183CrossRefGoogle Scholar
  17. 17.
    Hammatt, N., Blackhall, N W, and Davey, M R (1991) Variation in the DNA content of Glycine species. J Exp Bot. 42, 659–665CrossRefGoogle Scholar
  18. 18.
    Vos, P, Hogers, R, Bleeker, M., Reijans, M., van de Lee, T, Homes, M, et al (1995) AFLP: a new technique for DNA fingerprinting Nucleic Acids Res 23, 4407–4414PubMedCrossRefGoogle Scholar
  19. 19.
    Williams, J G K, Kubehk, A. R., Livark, K. J., Rafalski, J. A., and Fingey, S V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers Nucleic Acids Res 18, 6531–6535PubMedCrossRefGoogle Scholar
  20. 20.
    Vaccino, P., Accerbi, M, and Carbelhni, M. (1993) Cultivar identification in Triticum aestivum using highly polymorphic RFLP probes Theor Appl Genet 86, 833–836.CrossRefGoogle Scholar
  21. 21.
    Powell, W., Machray, G. C., and Provan, J. (1996) Polymorphism revealed by simple sequence repeats. Trends Plant Sci 1, 215–222.Google Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  1. 1.Department of Life SciencesUniversity of NottinghamNottinghamUK

Personalised recommendations

Cite protocol

Buy options