In Vitro Propagation of Succulent Plants

  • Jill Gratton
  • Michael F. Fay
Part of the Methods In Molecular Biology™ book series (MIMB, volume 111)


Maintenance of collections of succulent plants can be problematic, because many of these species are very susceptible to rots caused by bacteria and fungi. Rooting and establishment of cuttings can also be difficult. Tissue-culture techniques have been applied to a wide range of succulents, and the relevant literature has recently been reviewed (1,2). Here we describe methods for the micropropagation of cacti and other succulents that have been developed at Kew for overcoming the problems mentioned above.


Sodium Hypochlorite Surface Sterilization Semisolid Medium Succulent Plant Naphthyl Acetic Acid 
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  1. 1.
    Fay, M F. and Gratton, J. (1992) Tissue culture of cacti and other succulents—a literature review and report of micropropagation at Kew Bradleya 10, 33–48Google Scholar
  2. 2.
    Fay, M F., Gratton, J, and Atkinson P J. (1995) Tissue culture of succulent plants—an annotated bibliography. Bradleya 13, 38–42.Google Scholar
  3. 3.
    Murashige, T and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15, 473–497CrossRefGoogle Scholar
  4. 4.
    Mauseth, J. D and Halperin, W (1975) Hormonal control of organogenesis in Opuntia polyacantha (Cactaceae). J Am Bot 62, 869–877CrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Jill Gratton
    • 1
  • Michael F. Fay
    • 2
  1. 1.Royal Botanic GardensRichmondUK
  2. 2.Department of Life SciencesUniversity of NottinghamNottinghamUK

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