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Animal Models to Study Chemokine Receptor Function In Vivo

Mouse Models of Allergic Airway Inflammation
  • Clare M. Lloyd
  • Jose-Carlos Gutierrez-Ramos
Part of the Methods in Molecular Biology™ book series (MIMB, volume 239)

Abstract

Animal models using guinea pigs, monkeys, rats, and mice have been employed to study the pathogenesis of asthma. More recently, mouse models of allergic lung disease have been used to dissect the complex pathophysiological mechanisms underlying the asthma phenotype. This increase in usage of mouse models stems from the range of tools available for outlining functional pathways for individual cells and mediators. Genetic technologies allow us to manipulate the expression of particular molecules of interest and, therefore, it is possible to dissect inflammatory pathways to investigate the functional roles of particular mediators or cells. Mice can be induced to display a range of the pathophysiological features that are hallmarks of the human disease, including inflammatory cell recruitment to the lung, mucus secretion from the bronchoepithelial surface, serum IgE, and IgG2a, accompanied by a Th2 cytokine profile. Importantly, these pathophysiological indicators are generally accompanied by changes in lung function, allowing correlation between changes in cell or mediator expression and lung physiology.

Keywords

Complete RPMI Allergic Airway Inflammation Airway Hyperreactivity Tracheal Cannula Column Buffer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Murphy, K. M., Heimberger, A. B., and Loh, D. Y. (1990) Induction by antigen of intrathymic apoptosis of CD4+CD8+TCRlo thymocytes in vivo. Science 250, 1720–1723.PubMedCrossRefGoogle Scholar
  2. 2.
    Townsend, M. J., Fallon, P. G., Matthews, D. J., Smith, P., Jolin, H. E., and McKenzie, A. N. (2000) IL-9-deficient mice establish fundamental roles for IL-9 in pulmonary mastocytosis and goblet cell hyperplasia but not T cell development. Immunity 13, 573.PubMedCrossRefGoogle Scholar
  3. 3.
    Drazen, J. M., Finn, P. W., and De Sanctis, G. T. (1999) Mouse models of airway responsiveness: physiological basis of observed outcomes and analysis of selected examples using these outcome indicators. Annu. Rev. Physiol. 61, 593.PubMedCrossRefGoogle Scholar
  4. 4.
    Lloyd, C. M., Gonzalo, J. A., Coyle, A. J., and Gutierrez-Ramos, J. C. (2001) Mouse models of allergic airway disease. Adv. Immunol. 77, 263–295.PubMedCrossRefGoogle Scholar
  5. 5.
    Campbell, E. M. and Lukacs, N. W. (2000) Murine models of airway inflammation. Methods Mol. Biol. 138, 295.PubMedGoogle Scholar
  6. 6.
    Lloyd, C. M., Gonzalo, J. A., Nguyen, T., et al. (2001) Resolution of bronchial hyperresponsiveness and pulmonary inflammation is associated with IL-3 and tissue leukocyte apoptosis. J. Immunol. 166, 2033.PubMedGoogle Scholar
  7. 7.
    Lloyd, C. M., Delany, T., Nguyen, T., et al. (2000) CC Chemokine receptor (CCR)3/Eotaxin is followed by CCR4/monocyte-derived chemokine in mediating pulmonary T helper lymphocyte type 2 recruitment after serial antigen challenge in vivo. J. Exp. Med. 191, 265.PubMedCrossRefGoogle Scholar
  8. 8.
    Gonzalo, J. A., Pan, Y., Lloyd, C. M., et al. (1999) Mouse monocyte-derived chemokine is involved in airway hyperreactivity and lung inflammation. J. Immunol. 163, 403.PubMedGoogle Scholar
  9. 9.
    Gonzalo, J. A., Lloyd, C. M., Peled, A., et al. (2000) Critical involvement of the chemotactic axis CXCR4/stromal cell-derived factor-1 alpha in the inflammatory component of allergic airway disease. J. Immunol. 165, 499.PubMedGoogle Scholar
  10. 10.
    Gonzalo, J.-A., Lloyd, C. M., Kremer, L., et al. (1996) Eosinophil recruitment to the lung in a murine model of allergic inflammation. The role of T cells, chemokines and adhesion receptors. J. Clin. Invest. 98, 2332.PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press Inc., Totowa, NJ 2004

Authors and Affiliations

  • Clare M. Lloyd
    • 1
  • Jose-Carlos Gutierrez-Ramos
    • 2
  1. 1.Leukocyte Biology Section, Division of Biomedical Sciences, Faculty of MedicineImperial CollegeLondonUK
  2. 2.Millennium Pharmaceuticals Inc.Cambridge

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