Summary
Inositide-specific phospholipase C (PLC) β can be activated by the Gq family of Gα subunits and by the Gβγ subunits of heterotrimeric G proteins. PLC catalyzes the hydrolysis of phosphotidylinositol 4,5-bisphosphates (PIP2) to inositol trisphosphate (IP3) and diacylglycerol (DAG). This chapter describes a cell-based assay system to determine the activity of PLC by monitoring the levels of IPs in cultured cells. This assay system can be used to examine the activation of G protein-coupled receptors (GPCRs), coupling of GPCRs to G proteins, and the interactions of G proteins to PLCβ.
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© 2004 Humana Press Inc.
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Liu, B., Wu, D. (2004). Analysis of G Protein-Mediated Activation of Phospholipase C in Cultured Cells. In: Smrcka, A.V. (eds) G Protein Signaling. Methods in Molecular Biology™, vol 237. Humana Press. https://doi.org/10.1385/1-59259-430-1:99
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DOI: https://doi.org/10.1385/1-59259-430-1:99
Publisher Name: Humana Press
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