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Measurement of F4-Neuroprostanes by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization

  • Nathalie Bernoud-Hubac
  • L. Jackson RobertsII
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

Oxidative damage has been strongly implicated in the pathogenesis of a number of neurodegenerative diseases including Alzheimer’s disease (AD), Parkinson’s disease (PD), amyotrophic lateral sclerosis (ALS), and Huntington’s disease (HD) (1, 2, 3, 4, 5, 6, 7, 8, 9). Several years ago, we reported the discovery of the formation of F2-isoprostanes (F2-IsoPs), which are prostaglandin F2-like compounds formed in vivo nonenzymatically as products of free radical-induced oxidation of arachidonic acid (10). More recently we described the formation of IsoP-like compounds in vivo from free radical-induced oxidation of docosahexaenoic acid (DHA) (5). DHA is a fatty acid that is uniquely enriched in the brain, particularly the gray matter, where it comprises approx 25–35% of the total fatty acids in aminophospholipids (11,12). Our motive for exploring whether IsoP-like compounds are formed from oxidation of DHA derives from our hypothesis that measurement of such compounds may provide a unique and sensitive biomarker of oxidative neuronal injury in the brain. Accordingly, we have termed these compounds neuroprostanes (NPs).

Keywords

Amyotrophic Lateral Sclerosis Peroxyl Radical Trimethylsilyl Ether Plastic Test Tube Pentafluorobenzyl Bromide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc.,Totowa, NJ 2003

Authors and Affiliations

  • Nathalie Bernoud-Hubac
    • 1
  • L. Jackson RobertsII
    • 1
  1. 1.Departments of Pharmacology and MedicineVanderbilt UniversityNashville

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