Measurement of Isofurans by Gas Chromatography-Mass Spectrometry/Negative Ion Chemical Ionization
Many methods have been developed to assess oxidative stress status in vivo, which include products of lipid, protein, and DNA oxidation. However, it has long been recognized that most of these methods are unreliable because they lack specificity, sensitivity, or are too invasive for human investigation (1). In 1990, Roberts and Morrow described formation of prostaglandin F2-like compounds, F2-isoprostanes (F2-IsoPs), in vivo by nonen-zymatic free radical-induced peroxidation of arachidonic acid (2). Measurement of F2-IsoPs by gas chromatography-mass spectrometry/negative ion chemical ionization (GC-MS/NICI) has since emerged as one of the most sensitive and reliable approaches to assess lipid peroxidation and oxidative stress status in vivo (3,4).
KeywordsArachidonic Acid Oxygen Tension Oxidative Stress Status Silica Film Phosphomolybdic Acid
- 2.Morrow, J. D., Hill, K. E., Burk, R. F., Nammour, T. M., Badr, K. F., and Roberts, L.J., 2nd (1990) A series of prostaglandin F2-like compounds are produced in vivo in humans by a non-cyclooxygenase, free radical-catalyzed mechanism. Proc. Natl. Acad. Sci. USA 87, 9383–9387.PubMedCrossRefGoogle Scholar
- 7.Fessel, J. P., Porter, N. A., Moore, K. P., Sheller, J. R., and Roberts, L. J., II (2002) Discovery of lipid peroxidation products formed in vivo with a substituted tetrahydrofuran ring (isofurans) that are favored by increased oxygen tension. Proc. Natl. Acad. Sci. USA 99, 16,713–16,718.PubMedCrossRefGoogle Scholar