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Detection of Reactive Oxygen Species by Flow Cytometry

  • Alexander Christov
  • Ladan Hamdheydari
  • Paula Grammas
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

Reactive oxygen species (ROS) are a family of molecules including molecular oxygen and its derivatives produced in all aerobic cells. Extensive production of ROS has been implicated in the oxidation of biological macromolecules, such as DNA, proteins, carbohydrates, and lipids. This condition has commonly been referred to as oxidant stress. Oxidant stress is involved in the pathogenesis of many cardiovascular diseases and is closely related to vascular endothelial dysfunction (1). Endothelial dysfunction includes altered anticoagulant and antiinflammatory properties of the endothelium, impaired modulation of vascular growth, and dysregulation of vascular remodeling (2).

Keywords

Reactive Oxygen Species Reactive Oxygen Species Level Intracellular Reactive Oxygen Species Brain Endothelial Cell Endothelial Cell Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc.,Totowa, NJ 2003

Authors and Affiliations

  • Alexander Christov
    • 1
    • 2
  • Ladan Hamdheydari
    • 1
    • 2
  • Paula Grammas
    • 1
    • 2
  1. 1.Department of PathologyUniversity of Oklahoma Health Sciences CenterOklahoma City
  2. 2.Oklahoma Center for NeuroscienceOklahoma City

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