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Single-Cell Gel Electrophoresis or Comet Assay of Intestinal Epithelial Cells Using Manual Scoring and Ridit Analysis

  • Mark M. Huycke
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

The single-cell gel electrophoresis or comet assay was initially reported in 1984 as a novel technique to directly visualize DNA damage in individual cells (1). The method was modified by adding strong alkaline conditions to denature genomic DNA and detect alkali labile lesions, greatly improving sensitivity for single-strand breaks (2,3). This simple assay has come into widespread use because of its sensitivity, need for few cells, applicability to nearly all eukaryotic cells, and ease in processing numerous samples (4).

Keywords

Comet Assay Comet Tail Ethidium Bromide Solution Comet Tail Length Ridit Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

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Copyright information

© Humana Press Inc.,Totowa, NJ 2003

Authors and Affiliations

  • Mark M. Huycke
    • 1
    • 2
    • 3
  1. 1.The Muchmore Laboratories for Infectious Diseases ResearchUniversity of Oklahoma Health Sciences CenterOklahoma City
  2. 2.Research Service, Department of Veterans Affairs Medical CenterUniversity of Oklahoma Health Sciences CenterOklahoma City
  3. 3.Department of MedicineUniversity of Oklahoma Health Sciences CenterOklahoma City

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