Abstract
In the postgenomic era, large-scale functional genomic approaches are necessary for converting sequence information into functional information. A para-genetic approach, called virus-induced gene silencing (VIGS), offers a rapid means of gaining insight into gene function in plants. VIGS system could be used to suppress endogenous gene expression by infecting plants with a recombinant virus vector (VIGS vector) carrying host-derived sequence. Here, we describe the use of tobacco rattle virus (TRV)-based VIGS technique to study gene function in Nicotiana benthamiana and tomato.
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© 2003 Humana Press Inc., Totowa, NJ
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Dinesh-Kumar, S.P., Anandalakshmi, R., Marathe, R., Schiff, M., Liu, Y. (2003). Virus-Induced Gene Silencing. In: Grotewold, E. (eds) Plant Functional Genomics. Methods in Molecular Biology™, vol 236. Humana Press. https://doi.org/10.1385/1-59259-413-1:287
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DOI: https://doi.org/10.1385/1-59259-413-1:287
Publisher Name: Humana Press
Print ISBN: 978-1-58829-145-5
Online ISBN: 978-1-59259-413-9
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