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Plant Functional Genomics pp 221–240Cite as

Gene and Enhancer Traps for Gene Discovery

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 236))

Abstract

Gene traps and enhancer traps provide a valuable tool for gene discovery. With this system, genes can be identified based solely on the expression pattern of an inserted reporter gene. The use of a reporter gene, such as β-glucuoronidase (GUS), provides a very sensitive assay for the identification of tissue- and cell-type specific expression patterns. In this chapter, protocols for examining and documenting GUS reporter gene activity in individual lines are described. Methods for the amplification of sequences flanking transposant insertions and subsequent molecular and genetic characterization of individual insertions are provided.

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Author information

Authors and Affiliations

  1. Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, CA

    Marcela Rojas-Pierce BS & Patricia S. Springer PhD

Authors
  1. Marcela Rojas-Pierce BS
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  2. Patricia S. Springer PhD
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Editor information

Editors and Affiliations

  1. Department of Plant Biology and Plant Biotechnology Center, The Ohio State University, Columbus, OH

    Erich Grotewold

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© 2003 Humana Press Inc., Totowa, NJ

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Rojas-Pierce, M., Springer, P.S. (2003). Gene and Enhancer Traps for Gene Discovery. In: Grotewold, E. (eds) Plant Functional Genomics. Methods in Molecular Biology™, vol 236. Humana Press. https://doi.org/10.1385/1-59259-413-1:221

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  • DOI: https://doi.org/10.1385/1-59259-413-1:221

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-145-5

  • Online ISBN: 978-1-59259-413-9

  • eBook Packages: Springer Protocols

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