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Vaccine Protocols pp 313–334Cite as

Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment

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Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 87))

Abstract

New or improved vaccines require testing before they are licensed for use in the general population. Although toxicity and immunological data can be obtained from Phase I human trials, in the vast majority of cases it is not possible to demonstrate efficacy by challenging vaccine recipients with the specific infectious agent. Therefore, prior to large-scale production of vaccines and their assessment in Phase II and III clinical trials, there is a requirement for in vivo studies that can predict the level of protection that may be expected from a vaccine. Although many animal models are available for infectious disease studies, they all rely on the animal’s own immune system for the generation of the protective responses, and these may not reflect the immunological responses generated in humans. The route of challenge that must be used in order to achieve infection is often not the same as the one that occurs in human disease, and this further confounds the relevance of the animal model. In addition, some infections are specific to humans, and it is sometimes impossible to find a suitable animal species that human pathogens will infect. This may be because of the lack of appropriate receptors, the presence of a functional immune system able to eliminate the pathogen, or the fact that models cannot always be relied on to re-create the normal pathophysiology. In some cases, this resistance to infection can be overcome by the development of a transgenic animal in which the appropriate receptors are expressed. The development of transgenic models has been directed toward genetic disorders or dissection of the immune system by gene-knockout technology.

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References

  1. McCune, J. M., Namikawa, R., Kaneshima, H., Shultz, L. D., Lumberman, M., and Welshman, I. L. (1988) The SCID-Hu mouse: murine model for the analysis of human hematolymphoid differentiation and function. Science 241, 1632–1639.

    Article  PubMed  CAS  Google Scholar 

  2. Mosier, D. E. (1990) Immunodeficient mice xenografted with human lymphoid cells: new models for in vivo studies of human immunobiology and infectious diseases. J. Clin. Immunol. 10, 185–191.

    Article  PubMed  CAS  Google Scholar 

  3. Mosier, D. E., Gulizia, R. J., MacIsaac, P., Mathieson, B. J., Smith, G., Hu, S. L., et al. (1992) Evaluation of gpl60 vaccinees in the hu-PBL-SCID mouse model. AIDS Res. Hum. Retrovir. 8, 1387.

    PubMed  CAS  Google Scholar 

  4. Mosier, D. E., Gulizia, R. J., Baird, S. M., Wilson, D. B., Spector, D. H., and Spector, S. A. (1991) Human immunodeficiency virus infection of human-PBL-SCID mice. Science 251, 791–794.

    Article  PubMed  CAS  Google Scholar 

  5. Torbett, B. E., Picchio, G., and Mosier, D. E. (1991) Hu-PBL-SCID mice-a model for human immune function, AIDS, and lymphomagenesis. Immunol. Rev. 124, 139–164.

    Article  PubMed  CAS  Google Scholar 

  6. Mosier, D. E., (1996) Viral pathogenesis in hu-PBL-SCID mice. Semin. Immunol. 8, 255–262.

    Article  PubMed  CAS  Google Scholar 

  7. Levine, B., Hardwick, J. M., Trapp, B. D., Crawford, T. O., Bollinger, R. C, and Griffin, D. E. (1991) Antibody-mediated clearance of alphavirus infection from neurons. Science 254, 856–860.

    Article  PubMed  CAS  Google Scholar 

  8. Baiocchi, R. A. and Caligiuri, M. A. (1994) Low dose interleukin 2 prevents the development of Epstein-Barr virus (EBV) associated lymphoproliferative disease in scid/scid mice reconstituted i.p. with EBV-seropositive human peripheral blood lymphocytes. Proc. Natl. Acad. Sci. USA 91, 5577–5581.

    Article  PubMed  CAS  Google Scholar 

  9. Berney, T., Damarism Molano, R., Pileggi, A., Cattan, P., Li, H., Ricordi, C., and Inverardi, L. (2001) Patterns of engraftment in different strains of mice reconstituted with human peripheral blood lymphocytes. Transplantation 72, 133–140.

    Google Scholar 

  10. Carballido, J. M., Namikawa, R., Carballido-Perrig, N., Antonenko, S., Roncarolo, M. G., and de Vries, J. E. (2000) generation of primary antigen-specific human T-and B-cell responses in immunocompetent SCID-hu mice. Nat. Med. 6, 103–106.

    Article  PubMed  CAS  Google Scholar 

  11. Zhang, Z., Jin, L., Champion, G., Seydel, K. B., and Stanley S. L. (2001) Shigella infection in a SCID mouse-human intestinal xenograft model: a role for neutrophils in containing bacterial dissemination in human intestine. Infect. Immun. 69, 3240–3247.

    Article  PubMed  CAS  Google Scholar 

  12. Hall, G. A, Hodgeson, A. E, Leach, S. A, Dennis, M. J., Cawthraw, S., and Newell, D. (1999) Experience with intestinal xenografts to model Campylobacter infection. European Soc. Vet. Pathol. 17th meeting, Nantes, p. 167.

    Google Scholar 

  13. Scaramuzzino, D. A., McNiff, J. M., and Bessen, D. E. (2000) Humanized in-vivo model for streptococcal impetigo. Inf. Immun. 68, 2880–2887.

    Article  CAS  Google Scholar 

  14. Bidanset, D. J., Ryback, R. J., Hartline, C. B., and Kern, E. R. (2001) Replication of human cytomegalovirus in severe combined immunodeficient mice implanted with human retinal tissue. J. Infect. Dis. 184, 192–195.

    Article  PubMed  CAS  Google Scholar 

  15. Ilan, E., Arazi, J., Nussbaum, O., Zauberman, A., Erin, R., Lubin, I., et al. (2002) The Hepatitis C virus (HCV)-trimera mouse: A model for evaluation of agents against HCV. I. Infect. Dis. 185, 153–161.

    Article  CAS  Google Scholar 

  16. Kish, T. M., Budgeon, L. R., Welsh, P. A., and Howett, M. K. (2001) Immunological characterization of human vaginal xenografts in immunocompromised mice: development of a small animal model for the study of HIV-1 infection. Am. J. Pathol. 159, 2331–2345.

    Article  PubMed  CAS  Google Scholar 

  17. Segall, H., Lubin, I., Marcus, H., Canaan, A., and Reisner, Y. (1996) Generation of primary antigen-specific human CTL in human/mouse radiation chimera. Blood 88, 721–730.

    PubMed  CAS  Google Scholar 

  18. Marusic, C., Rizza, Lattanzi, L., Mancini, C., Spada, M., Bellardelli, F., et al. (2001) Chimeric plant virus particles as immunogens for inducing murine and human immune responses against HIV-1. J. Virol. 75, 8434–8439.

    Article  PubMed  CAS  Google Scholar 

  19. Delhem, N., Hadida, F., Gorochov, G., Carpentier, F., de Cavel, J. P., Andreani, J. F., et al. (1998) Primary Th1 cell immunization against HIVgp160 in SCID-hu mice co-engrafted with peripheral blood lymphocytes and skin. J. Immunol. 161, 2060–2069.

    PubMed  CAS  Google Scholar 

  20. Duchosal, M. A., Fuzzati-Armentero, M. T., Baccala, R., Layer, A., Gonzalez-Quintial, R., Leturq, M. R., et al. (2000) Human adult tonsil xenotransplantation into SCID mice for studying human immune responses and B-cell lymphomagenesis. Exp. Hematol. 28, 177–192.

    Article  PubMed  CAS  Google Scholar 

  21. Albert, S. E., McKerlie, C., Pester, A., Edgell, B. J., Carlyle, J., Petric, M., et al. (1997) Time-dependent induction of protective anti-influenza immune responses. J. Immunol. 159, 1393–1403.

    PubMed  CAS  Google Scholar 

  22. Holscher, C., Hasch, G., Joswig, N., Stauffer, U., Muller, U., and Mossman, H. (1999) Long-term substitution and specific immune responses after transfer of bovine peripheral blood lymphocytes into severe combined immunodeficient mice. Vet. Immunol. Immunopathol. 70, 67–83.

    Article  PubMed  CAS  Google Scholar 

  23. Murphey, W. J., Funarkoski, S., Fanslow, W. C., Rager, H. C., Taub, D. D., and Longo, D. L. (1999) CD40 stimulation promotes human secondary immunoglobulin reponses in HuPBL-SCID chimeras. Clin. Immunol. 90, 22–27.

    Article  Google Scholar 

  24. Sandhu, J., Shpitz, B., Gallinger, S., and Hozumi, N. (1994) Human primary immune response in SCID mice engrafted with human peripheral blood lymphocytes. J. Immunol. 152, 3806–3813.

    PubMed  CAS  Google Scholar 

  25. Tournoy, K. G., Depraetere, S., Pauwels, R. A., and Leroux-Roels, G. G. (2000) Mouse strain and conditioning regimen determine survival and function of human leucocytes in immunodeficient mice. Clin. Exp. Immunol. 119, 231–239.

    Article  PubMed  CAS  Google Scholar 

  26. Mead, J. R., Arrowood, M. J., Healey, M. C., and Sidwell, R. W. (1991) Cryptosporidial infections in SCID mice reconstituted with human or murine lymphocytes. J. Protozool. 38, 59S–61S.

    PubMed  CAS  Google Scholar 

  27. Sarin, P. S., Talmadge, J. E., Heseltine, P., Murcar, N., Gendelman, H. E., Coleman, R., et al. (1999) Booster immunization of HIV-1 negative volunteers with HGP-30 vaccine induces protection against HIV-1 virus challenge in SCID mice. Vaccine 17, 64–71.

    Article  PubMed  CAS  Google Scholar 

  28. Nguyen, H., Hay, J., Mazzuli, T., Gallinger, S., Sandhu, J., Teng, Y-T. A., and Hozumi, N. (2000) Efficient generation of respiratory syncitial virus (RSV)-neutralizing human MoAbs via human peripheral blood lymphocyte (huPBL)-SCID mice and scFv phage display libraries. Clin. Exp. Immunol. 122, 85–93.

    Article  PubMed  CAS  Google Scholar 

  29. Baiocchi, R. A., Ward, J. S., Carrodeguas, L., Eisbeis, C. F., Peng, R., Roychowdhury, S., et al. (2001) GM-CSF and IL-2 induce specific cellular immunity and provide protection against Epstein-Barr virus lymphoproliferative disorder. J. Clin. Investig. 108, 887–894.

    PubMed  CAS  Google Scholar 

  30. Gupta, M., Mahanty, S., Bray, M., Ahmed, R., and Rollin, P. E. (2001) Passive transfer of antibodies protects immunocompetent and immunodeficient mice against lethal Ebola virus infection without complete inhibition of viral replication. J. Virol. 75, 4649–4654.

    Article  PubMed  CAS  Google Scholar 

  31. Gaudin, M. C., Allaway, G. P., Olson, W. C., Weir, R., Maddon, P. J., and Koup, R. A. (1998) CD4-immunoglobulin G2 protects Hu-PBL-SCID mice against challenge by primary human immunodeficiency virus type 1 isolates. J. Virol. 72, 3475–3478.

    Google Scholar 

  32. Fowler, K., McBride, B. W., Turnbull, P. C., and Baillie, L. W. (1999) Immune correlates of protection against anthrax. J. Appl. Microbiol. 87, 305.

    Article  PubMed  Google Scholar 

  33. Bosma, M. J. and Carroll, A. M. (1991) The SCID mouse mutant: definition, characterization, and potential uses. Annu. Rev. Immunol. 9, 323–350.

    Article  PubMed  CAS  Google Scholar 

  34. Jhappan, C., Morse, H. C., Fleischmann, R. D., Gottesman, M. M, and Merlino, G. (1997) DNA-PKcs: a T-cell tumour suppressor encoded at the mouse scid locus. Nat. Genet. 17, 483–486

    Article  PubMed  CAS  Google Scholar 

  35. Hinkley, K. S., Chiasson, R. J., Prior, T. K., and Riggs, J. E. (2002) Age-dependent increase of peritoneal B-1b B-cells in SCID mice. Immunology 105, 196–203.

    Article  PubMed  CAS  Google Scholar 

  36. Garcia, S., Dadaglio, G., and Gougeon, M. L. (1997) Limits of the Human-PBL-SCID mice model: severe restrictions of the Vβ T-cell repertoire of engrafted human T-cells. Blood 89, 329–336.

    PubMed  CAS  Google Scholar 

  37. Nonoyama, S., Smith, F. O., Bernstein, I. D., and Ochs, H. (1993) Strain-dependent leakiness of mice with severe combined immune deficiency. J. Immunol. 150, 3817–3824.

    PubMed  CAS  Google Scholar 

  38. Anderson, M. R. and Tary-Lehmann, M. (2001) Staphylococcal enterotoxin-B-induces lethal shock in mice is T-cell dependent but disease susceptibility is defined by the non T-cell compartment. Clin. Immunol. 98, 85–94.

    Article  PubMed  CAS  Google Scholar 

  39. MacDougall, J. R., Croy, B. A., Chapeau, C., and Clark, D. A. (1990) Demonstration of a splenic cytotoxic effector cell in mice of genotype SCID/SCID.BG/BG. Cell. Immunol. 130, 106–117.

    Article  PubMed  CAS  Google Scholar 

  40. Mosier, D. E., Stell, K. L., Gulizia, R. J., Torbett, B. E., and Gilmore, G. L. (1993) Homozygous scid/scid-beige/beige mice have low levels of spontaneous or neonatal T-cell-induced B-cell generation. J. Exp. Med. 177, 191–194.

    Article  PubMed  CAS  Google Scholar 

  41. Froidevaux, S. and Loor, F. (1991) A quick procedure for identifying doubly homozygous immunodeficient scid beige mice. J. Immunol. Methods 137, 275–279.

    Article  PubMed  CAS  Google Scholar 

  42. Shultz, L. D., Schweitzer, P. A., Christianson, S. W., Gott, B., Schweitzer, I. B., Tennent, B., Mckenna, S., Mobraaten, L., Rajan, T. V., and Geiner, D. L. (1995) Multiple defects in innate and adaptive immunologic function in NOD/LtSz-scid mice. J. Immunol. 154, 180–191.

    PubMed  CAS  Google Scholar 

  43. Greiner, D. L. and Shultz, L. D. (1998) The use of NOD/LtSz-scid/scid mice in biomedical research, in NOD Mice and Related Strains: Research Applications in Diabetes, AIDS, Cancer and Other Diseases. (Leiter, E. and Athinson, M., eds.) Landes Bioscience, Austin, TX pp. 173–203.

    Google Scholar 

  44. Dick, J. E., Guenechea, G., Gan, O. I., and Dorrell, C. (2001) In vivo dynamics of human stem cell repopulation in NOD/SCID mice. Ann. NY Acad. Sci. 938, 184–190.

    Article  PubMed  CAS  Google Scholar 

  45. Lapidot, T. (2001) Mechanism of human stem-cell migration and repopulation of NOD/SCID and B2mnull mice: The role of SDF-1/CXCR4 interactions. Ann. NY Acad. Sci. 938, 83–95.

    Article  PubMed  CAS  Google Scholar 

  46. Kollet, O., Peled, A., Byk, T., Ben-Hur, H., Greiner, D., Shultz, L., and Lapidot, T. (2000) β2 microglobin-deficient (B2mnull) NOD/SCID mice are excellent recipients for studying human stem cell function. Blood 95, 3102–3105.

    PubMed  CAS  Google Scholar 

  47. Kerre, T. C., De Smet, G., De Smet, M., Zippelius, A., Pittet, M. J., Langerak, A. W., de Bosscher, J., Offner, F., Vanderkerchove, B., and Plum, J. (2002) Adapted NOD/SCID model supports development of phenotypically and functionally mature T-cells from human umbilical cord blood CD34(+) cells. Blood 99, 1620–1626.

    Article  PubMed  CAS  Google Scholar 

  48. Hogan, C. J., Shpall, E. J., McNulty, O., McNiece, I., Dick, J. E., Shultz, L. D., and Keller, G. (1997) Engraftment and development of human CD34(+)-enriched cells from umbilical cord blood in NOD/LtSz-scid/scid mice. Blood 90, 85–96.

    PubMed  CAS  Google Scholar 

  49. Hogan, C. J., Shpall, E. J., McNiece, I., and Keller, G. (1997) Multilineage engraftment in NOD/LtSz-scid/scid mice from mobilized human CD34(+) peripheral blood progenitor cells. Biol. Blood Marrow Transplant 3, 236–246.

    PubMed  CAS  Google Scholar 

  50. Shinkai, Y., Rathbun, G., Lam, K.-P., Oltz, E. M., Stewart, V., Mendelsohn, M., et al. (1992) RAG-2-Deficient mice lack mature lymphocytes owing to inability to initiate V(D)J rearrangement. Cell 68, 855–867.

    Article  PubMed  CAS  Google Scholar 

  51. Shultz, L. D., Lang, P. A., Christianson, S. W., Gott, B., Lyons, B., Umeda, S., et al. (2000) NOD/LtSz-Rag/null mice: an immunodeficient and radio-resistant model for engraftment of human hematolymphoid cells, HIV infection and adoptive transfer of NOD mouse diabetogenic T-cells. J. Immunol. 164, 2496–2507.

    PubMed  CAS  Google Scholar 

  52. Aaberge, I. S., Michaelsen, T. E., Rolstad, A. K., Groeng, E. C., Solberg, P., and Lovik, M. (1992) SCID-Hu mice immunized with a pneumococcal vaccine produce specific human antibodies and show increased resistance to infection. Infect. Immunol. 60, 4146–4153.

    CAS  Google Scholar 

  53. He, X., Weyand, C. M., Goronzy, J. J., Zhong, W., and Stuart, J. M. (2002) Bi-directional modulation of T-cell-dependent antibody production by prostaglandin E(2) Int. Immunol. 14, 69–77.

    Article  PubMed  CAS  Google Scholar 

  54. Wedderburn, L. R., Jeffery, R., White, H., Patel, A., Vaarsini, H., Lind, D., et al. (2001) Autologous stem cell transplantation for paediatric-onset polyarthritis nodosa: changes in autoimmune phenotype in the context of reduced diversity of the T-and B-cell repertoires and evidence for reversion from CD45RO(+) to RA(+) phenotype. Rheumatology 40, 1299–1307.

    Article  PubMed  CAS  Google Scholar 

  55. Giorgi, J. V., Hausner, M. A., and Hultin, L. E. (1999) Detailed immune phenotype of CD8+ memory cyotoxic T-lymphocytes (CTL) against HIV-1 with respect to expression of CD45RA/RO, CD62L and CD28 antigens. Immunol. Lett. 66, 105–110.

    Article  PubMed  CAS  Google Scholar 

  56. Roncarolo, M. G., Carballido, J. M., Rouleau, M., Namikawa, R., and de Vries, J. E. (1996) Human T-and B-cell functions in SCID-hu mice. Semin. Immunol. 8, 207–213.

    Article  PubMed  CAS  Google Scholar 

  57. Mosier, D. E., Gulizia, R. J., MacIsaac, P. D., Corey, L., and Greenberg, P. D. (1993) Resistance to human immunodeficiency virus 1 infection of SCID mice reconstituted with peripheral blood leukocytes from donors vaccinated with vaccinia gpl60 and recombinant gpl60. Proc. Natl. Acad. Sci. USA 90, 2443–2447.

    Article  PubMed  CAS  Google Scholar 

  58. Van Kuyk, R., Torbett, B. E., Gulizia, R. J., Leath, S., Mosier, D. E., and Koenig, S. (1994) Cloned human CD8+ T lymphocytes protect human peripheral blood leukocyte-severe combined immunodeficient mice from HIV-l infection by an HLA-unrestricted mechanism. J. Immunol. 153, 4826–4833.

    PubMed  Google Scholar 

  59. Williams, A., McBride, B. W., Hall, G., Fitzgeorge, R. B., and Farrar, G. H. (1995) Experimental legionnaires disease in SCID-Beige mice reconstituted with human leukocytes. J. Med. Microbiol. 42, 433–441.

    Article  PubMed  CAS  Google Scholar 

  60. Ono, M., Wada, Y., Wu, Y., Nemori, R., Jinbo, Y., Wang, H., et al. (1997) FP-21399 blocks HIV envelope protein-mediated membrane fussion and concentrates in lymph nodes. Nat. Biotechnol. 15, 343–348.

    Article  PubMed  CAS  Google Scholar 

  61. Di Fabio, S., Giannini, G., Lapenta, C., Spada, M., Binelli, A., Germinario, E., et al. (2001) Vaginal transmission of HIV in hu-SCID mice: a new model for the evaluation of vaginal microbicides. AIDS 15, 2231–2238.

    Article  PubMed  Google Scholar 

  62. Bahr, G. M., Darcissac, E. C., Casteran, N., Amiel, C., Cocude, C., Truong, M. J., et al. (2001) Selective regulation of human immunodeficiency virus-infected CD4(+) lymphocytes by a synthetic immunomodulator leads to potent virus suppression in vitro and in hu-PGL-SCID mice. J. Virol. 75, 6941–6952.

    Article  PubMed  CAS  Google Scholar 

  63. Miura, Y., Misawa, N., Maeda, N., Inagaki, Y., Tanaka, Y., Ito, M., et al. (2001) Critical contribution of tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) to apoptosis of human CD4+ T-cells in HIV-1 infected hu-PGL-NOD-SCID mice. J. Exp. Med. 193, 651–660.

    Article  PubMed  CAS  Google Scholar 

  64. Brooks, D. G. and Zack, J. A. (2002) Effect of latent human immunodeficiency virus infection on cell surface phenotype. J. Virol. 76, 1673–1681.

    Article  PubMed  CAS  Google Scholar 

  65. Mercer, D. F., Schiller, D. E., Elliott, J. F., Douglas, D. N., Hao, C., Rinfret, A., et al. (2001) Hepatitis C virus replication in mice with chimeric human livers. Nat. Med. 7, 890–891.

    Article  Google Scholar 

  66. Santos, R. A., Hatfield, C. C., Cole, N. L., Padilla, J. A., Moffat, J. F., Arvin, A. M., et al. (2000) Varicella-zoster virus escape mutant VZV-MSP exhibits an accelerated cell-to cell spread phenotype in both infected cell cultures and SCID-hu mice. Virology 275, 306–317.

    Article  PubMed  CAS  Google Scholar 

  67. Pletnev, A. G. (2001) Infectious cDNA clone of attenuated Langat tick-borne flavivirus (strain E5) and a 3′ deletion mutant constructed from it exhibit decreased neuroinvasiveness in immunodeficient mice. Virology 282, 288–300.

    Article  PubMed  CAS  Google Scholar 

  68. VanCott, J. L., McNeal, M. M., Flint, J., Bailey, S. A., Choi, A. H., and Ward R. L. (2001) Eur. J. Immunol. 31, 3380–3387.

    Article  PubMed  CAS  Google Scholar 

  69. Neyts, J. and De Clerq, E. (2001) Efficacy of 2-amino-7-(1,3-dihydroxy-2-propoxymethyl) purine for treatment of Vaccinia virus (Orthopoxvirus) infections in mice. Antimicrob. Agents. Chemother. 45, 84–87.

    Article  PubMed  CAS  Google Scholar 

  70. Yoshida, M., Watanabe, T., Usui, T., Matsunaga, Y., Shirai, Y., Yamori, M., et al. (2001) CD4 T-cells monospecific to ovalbumin produced by Escherichia coli can induce colitis upon transfer to Balb/c and SCID mice. Int. Immunol. 13, 1561–1570.

    Article  PubMed  CAS  Google Scholar 

  71. Wirtz, S., Becker, C., Blumberg, R., Galle, P. R., and Neurath, F. (2002) Treatment of T-cell-dependent experimental colitis in SCID mice by local administration of an adenovirus expressing LI-18 antisense m RNA. J. Immunol. 168, 411–420.

    PubMed  CAS  Google Scholar 

  72. Leriche-Guerin, K., Anderson, L. V., Wrogemann, K., Roy, B., Goulet, M., Tremblay, J. P. (2002) Dysferlin expression after normal myoblast transplantation in SCID and in SJL mice. Neuromuscul. Disord. 12, 167–173.

    Article  PubMed  CAS  Google Scholar 

  73. Hodgeson, A. E., McBride, B. W., Hudson, M. J., Hall, G., and Leach S. A. (1998) Experimental campylobacter infection and diarrhoea in immunodeficient mice. J. Med. Microbiol. 47, 799–809.

    Article  Google Scholar 

  74. Hondalus, M. K., Bardarov, S., Russell, R., Chan, J., Jacobs, W. R., and Bloom, B. (2000) Attenuation of and protection induced by a leucine auxotroph of Mycobacterium tuberculosis. Infect. Immun. 68, 2888–2898.

    Article  PubMed  CAS  Google Scholar 

  75. Smith, D. A., Parish, T., Stoker, N. G., and Bancroft, G. J. (2001) Characterization of auxotrophic mutants of Mycobacterium tuberculosis and their potential as vaccine candidate. Infect. Immun. 69, 1142–1150.

    Article  PubMed  CAS  Google Scholar 

  76. Winslow, G. M., Yager, E., Shilo, K., Volk, E., Reilly, A., and Chu, F. K. (2000) Antibody-mediated elimination of the obligate intracellular bacterial pathogen Erlichia chaffeensis during active infection. Infect. Immun. 68, 2187–2195.

    Article  PubMed  CAS  Google Scholar 

  77. Paciorkowski, N., Porte, P., Shultz, L. D., and Rajan, T. V. (2000) B1 B lymphocytes play a critical role in host protection against lymphatic filarial parasites. J. Exp. Med. 191, 731–735.

    Article  PubMed  CAS  Google Scholar 

  78. Morrison, L. A., Zhu, L., and Thebeau, L. G. (2001) Vaccine-induced serum immunoglobulin contributes to protection from herpes simplex virus type 2 genital infection in the presence of immune T-cells. J. Virol. 75, 1195–1204.

    Article  PubMed  CAS  Google Scholar 

  79. Derby, M. A., Alexander-Miller, M. A., Tse, R., and Berkofsky, J. A. (2001) High-avidity CTL exploit two complementary mechanisms to provide better protection against viral infection than low-avidity CTL. J. Immunol. 166, 1690–1697.

    PubMed  CAS  Google Scholar 

  80. Cao, T., Meuleman, P., Deambere, I., Sallberg, M., and Leroux-Roels, G. (2001) In vivo inhibition of anti-hepatitis B virus core antigen (HbcAg) immunoglobulin G production by HbcAg-specific CD4(+) Th1-type T-cell clones in a hu-PBL-NOD/SCID mouse model. J. Virol. 75, 11,449–11,456.

    Article  PubMed  CAS  Google Scholar 

  81. Hoft, D. F., Schnapp, A. R., Eickhoff, C. S., and Roodman, S. T. (2000) Involvement of CD4+ Th1 cells in systemic immunity protective against primary and secondary challenges with Trypanosma cruzi. Infect. Immun. 68, 197–204.

    Article  PubMed  CAS  Google Scholar 

  82. Zhong, W., Gern, L., Stehle, T., Museteanu, C., Kramer, M., Wallich, R., and Simon, M. M. (1999) Resolution of experimental and tick-borne Borrelia burgdorferi infection in mice by passive but not active immunization using recombinant OspC. Eur. J. Immunol. 29, 946–957.

    Article  PubMed  CAS  Google Scholar 

  83. Naito, S., Okada, Y., Takahashi, M., Kato, H., Taneichi, M., Ami, Y., et al. (2000) Anti-tetanus toxoid antibody production and protection against lethal doses of tetanus toxin in hu-PBL-SCID mice. Int. Arch. Allergy Immunol. 123, 149–154.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Centre for Applied Microbiology and Research, Health Protection Agency, Porton Down, Salisbury, Wiltshire, UK

    Michael J. Dennis

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  1. Centre for Applied Microbiology and Research, Health Protection Agency, Porton Down, Salisbury, Wiltshire, UK

    Andrew Robinson  & Michael J. Hudson  & 

  2. Department of Cellular and Molecular Medicine, St. George’s Hospital Medical School, London, UK

    Martin P. Cranage

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© 2003 Humana Press Inc., Totowa, NJ

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Dennis, M.J. (2003). Severe Combined Immunodeficient (SCID) Mice in Vaccine Assessment. In: Robinson, A., Hudson, M.J., Cranage, M.P. (eds) Vaccine Protocols. Methods in Molecular Medicine™, vol 87. Humana Press. https://doi.org/10.1385/1-59259-399-2:313

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  • DOI: https://doi.org/10.1385/1-59259-399-2:313

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-140-0

  • Online ISBN: 978-1-59259-399-6

  • eBook Packages: Springer Protocols

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