Abstract
Several different approaches have been used to identify protein kinase C (PKC) protein-binding partners. It should be noted that each method may be biased toward identifying a certain type of binding protein, possibly because the methods used emphasize different aspects of PKC-binding protein interactions. This chapter describes features of the overlay assay for identifying PKC-binding partners and briefly compares and contrasts this method with others. Because of the abundance of PKC-binding proteins (1), those cases in which functional relevance has been established are emphasized.
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Jaken, S. (2003). An Overlay Assay for Detecting Protein Kinase C-Binding Proteins and Substrates. In: Newton, A.C. (eds) Protein Kinase C Protocols. Methods in Molecular Biology™, vol 233. Humana Press. https://doi.org/10.1385/1-59259-397-6:359
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DOI: https://doi.org/10.1385/1-59259-397-6:359
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