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A Chemical Genetic Approach for the Identification of Direct Substrates of Protein Kinases

  • Protocol
Protein Kinase C Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 233))

Abstract

Protein kinases form one of the largest superfamily of enzymes that play pivotal roles in controlling almost every signaling pathway (1). Deregulated kinase activity thus leads to multiple diseases, including various forms of cancer (2), inflammatory and autoimmune diseases (3), neurodegenerative diseases (4,5), diabetes (6), and HIV infection (7). Signaling networks regulated by kinases are complex and highly interconnected. Additionally, many kinases display overlapping substrate specificities in vitro and can functionally compensate for each other in single gene knockout experiments (8,9). Therefore, unraveling of these pathways to dissect the role of any particular kinase (normal or oncogenic) has remained one of the major challenges ever since the first kinase was purified. Ideally, if the substrate of each kinase could be identified in a cell, it would provide a baseline for understanding the complex cellular functions and consequently also provide a blueprint for novel targets for drug discovery.

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Author information

Authors and Affiliations

  1. Genomics Institute of the Novartis Research Foundation, San Diego, CA

    Kavita Shah

  2. Department of Cellular and Molecular Pharmacology, University of California San Francisco, San Francisco, CA

    Kevan M. Shokat

  3. Department of Chemistry, University of California, Berkeley, Berkeley, CA

    Kevan M. Shokat

Authors
  1. Kavita Shah
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  2. Kevan M. Shokat
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Editor information

Editors and Affiliations

  1. Department of Pharmacology, University of California at San Diego, La Jolla, CA

    Alexandra C. Newton

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© 2003 Humana Press Inc., Totowa, NJ

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Shah, K., Shokat, K.M. (2003). A Chemical Genetic Approach for the Identification of Direct Substrates of Protein Kinases. In: Newton, A.C. (eds) Protein Kinase C Protocols. Methods in Molecular Biology™, vol 233. Humana Press. https://doi.org/10.1385/1-59259-397-6:253

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  • DOI: https://doi.org/10.1385/1-59259-397-6:253

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-068-7

  • Online ISBN: 978-1-59259-397-2

  • eBook Packages: Springer Protocols

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