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An Overview of High-Throughput Screening Systems for Enantioselective Enzymatic Transformations

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Directed Enzyme Evolution

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 230))

Abstract

The directed evolution of enantioselective enzymes (1,2) for use in organic synthesis constitutes an attractive alternative to traditional forms of asymmetric catalysis based on chiral transition metal complexes or catalytic antibodies. It involves the proper combination of molecular biological methods for random gene mutagenesis and gene expression coupled with appropriate high-throughput screening systems that allow the rapid determination of the enantiomeric purity of a chiral product. Typically, thousands of samples arising from the catalytic action of the evolved enzyme variants on a given substrate of interest need to be assayed within a reasonable time span, ideally within one day.

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Authors and Affiliations

  1. Max Planck Institut für Kohlenforschung, Mülheim/Ruhr, Germany

    Manfred T. Reetz

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  1. Manfred T. Reetz
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Editors and Affiliations

  1. California Institute of Technology, Pasadena, CA

    Frances H. Arnold

  2. University of Texas at Austin, Austin, TX

    George Georgiou

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© 2003 Humana Press Inc., Totowa, NJ

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Reetz, M.T. (2003). An Overview of High-Throughput Screening Systems for Enantioselective Enzymatic Transformations. In: Arnold, F.H., Georgiou, G. (eds) Directed Enzyme Evolution. Methods in Molecular Biology™, vol 230. Humana Press. https://doi.org/10.1385/1-59259-396-8:259

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  • DOI: https://doi.org/10.1385/1-59259-396-8:259

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-286-5

  • Online ISBN: 978-1-59259-396-5

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