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Detection and Selection of Lentiviral Vector-Transduced Cells

  • Protocol
Lentivirus Gene Engineering Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 229))

Abstract

Lentiviruses are members of a subgroup of enveloped Retroviridae. Lentivirus-based gene delivery vectors have gained popularity in gene therapy field because of their notable potentials in delivering and integrating transgenes into both mitotic active and inactive cells in vitro and in vivo, a characteristic that overcomes many of the barriers of therapeutic gene therapy application. In the past few years, different lentiviral vector systems have been developed based on primate and nonprimate lentiviruses, including human immunodeficiency virus type 1 (HIV-1), simian immunodeficiency virus (SIV), equine infectious anemia virus (EIAV), and feline immunodeficiency virus (FIV) (see refs. 1–4). However, HIV-1-based lentiviral vector remains the best-studied and well-developed lentiviral vector system that has shown high transduction efficiencies targeting many human and experimental animal cell types, including stem cells (embryonic and adult) (5–10), terminally differentiated somatic cells (i.e., neurons) (7,11,12), muscles (7,13,14), skin (15), liver (13,16,17), islet (18,19), lung epithelium (20,21), retina (22,23), primary T lymphocytes (24,25), and fetal tissues (21,26) (Table 1).

Table 1 Relative Efficiencies of Transduction of Various Cell Lines and Primary Cells with VSV-G Pseudotyped Lentiviral Vectorsa
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Author information

Authors and Affiliations

  1. Gene Therapy Program, Stanley S. Scott Cancer Center, Department of Medicine, Louisiana State University Health Sciences Center, LA

    Yan Cui

  2. Department of Molecular Genetics and Microbiology, Powell Gene Therapy Center, University of Florida, Gainesville, FL

    Lung-Ji Chang

  3. McKnight Brain Institute, University of Florida, Gainesville, FL

    Lung-Ji Chang

Authors
  1. Yan Cui
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  2. Lung-Ji Chang
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Editors and Affiliations

  1. Laboratory of Virology, Istituto Superiore di Sanitá, Rome, Italy

    Maurizio Federico

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© 2003 Humana Press Inc.

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Cui, Y., Chang, LJ. (2003). Detection and Selection of Lentiviral Vector-Transduced Cells. In: Federico, M. (eds) Lentivirus Gene Engineering Protocols. Methods in Molecular Biology™, vol 229. Humana Press. https://doi.org/10.1385/1-59259-393-3:69

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  • DOI: https://doi.org/10.1385/1-59259-393-3:69

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-091-5

  • Online ISBN: 978-1-59259-393-4

  • eBook Packages: Springer Protocols

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