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Lentivirus Gene Engineering Protocols pp 57–68Cite as

Detection and Titration of Lentivirus Vector Preparations

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 229))

Abstract

The accurate quantification of functional vector particles within a given vector preparation or stock is of fundamental importance to virtually all potential gene transfer applications for any viral vector of interest. This accuracy is especially critical if anticipated clinical applications will require scale-up of vector production or if multiple different vector stocks will be required for serial transductions of target cell populations over the course of a series of experiments. Standard notations designating the quantity of functional particles in a vector preparation include infectious units (i.u.) per milliliter and transducing units (TU) per milliliter (1). These units are interchangeable and represent the number of functional vector particles per volume of vector containing supernatant. The quantification of infectious or transducing units per unit volume allows transductions to be conducted based on the number of vector particles added per target cell and will therefore be standardized yielding more reproducible results. The numerical relationship between vector particles utilized per single target cell is designated as the multiplicity of infection (MOI). Establishing an appropriate MOI for a given transduction will, in part, depend on the desired transduction efficiency or, to an extent, the approximate number of chromosomal integrants desired per cell. Accurate vector stock titers will allow for the establishment of controlled transduction conditions and will, therefore, reduce experimental variability and simplify the interpretation of results obtained.

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Author information

Authors and Affiliations

  1. Division of Infectious Diseases, Department of Internal Medicine, University of Texas, Southwestern Medical Center at Dallas, Dallas, TX

    Joel Gatlin, Miguel Islas-Ohlmayer & J. Victor Garcia

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  1. Joel Gatlin
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  2. Miguel Islas-Ohlmayer
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  3. J. Victor Garcia
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Editor information

Editors and Affiliations

  1. Laboratory of Virology, Istituto Superiore di Sanitá, Rome, Italy

    Maurizio Federico

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© 2003 Humana Press Inc.

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Gatlin, J., Islas-Ohlmayer, M., Garcia, J.V. (2003). Detection and Titration of Lentivirus Vector Preparations. In: Federico, M. (eds) Lentivirus Gene Engineering Protocols. Methods in Molecular Biology™, vol 229. Humana Press. https://doi.org/10.1385/1-59259-393-3:57

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  • DOI: https://doi.org/10.1385/1-59259-393-3:57

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-091-5

  • Online ISBN: 978-1-59259-393-4

  • eBook Packages: Springer Protocols

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