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Extraction of DNA from Microdissected Archival Tissues

Protocol
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Part of the Methods in Molecular Biology™ book series (MIMB, volume 226)

Abstract

Many modern analytical methods require little material, and this has made feasible biochemical and molecular analyses of small tissue fragments, even individual cells, by microdissection of histological sections (1,2). Polymerase chain reaction (PCR) can potentially be applied to the analysis of single DNA molecules, as in the analysis of single haploid cells, such as spermatozoa (3). This sensitivity requires careful attention to technique and proper controls to avoid false-positive or other spurious results.

Keywords

Tungsten Wire Scalpel Blade Tungsten Needle Subsequent Polymerase Chain Reaction Polymerase Chain Reaction Thermal Cycler 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  1. 1.Department of PathologyGlasgow Royal InfirmaryGlasgowUK

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