In Situ Hybridization in Neural Tissues

  • Howard B. Gutstein
Part of the Methods in Molecular Biology™ book series (MIMM, volume 84)


Concomitant developments in molecular biology and neuroanatomy have permitted the development of techniques to visualize the expression of messenger RNA (mRNA) of interest in its neuroanatomical context and in a relatively quantitative manner. In situ hybridization can be performed using either radioactive or nonradioactive techniques and quantitated using specialized image analysis programs. This chapter will describe a sensitive radioactive method of in situ hybridization, with references given to other variants in technique.


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Copyright information

© Humana Press Inc. 2003

Authors and Affiliations

  • Howard B. Gutstein
    • 1
  1. 1.Departments of Anesthesiology and Molecular GeneticsThe University of Texas MD Anderson Cancer CenterHouston

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